Fig 1: Expression profiles of α-syn in lesions of patients with FCD IIb and TSC. (A) Moderate-to-strong α-syn immunoreactivity was observed in the neuropils of CTX but was neglectable in the neuronal cytoplasm. (B) Representative image showing weak α-syn signal in the neuropils of FCD IIb lesions and moderate α-syn immunoreactivity in DN (double arrow) and BC (arrow) of FCD IIb lesions. (C) Strong but less of α-syn positive signal was observed in the neuropils of TSC lesions, and α-syn immunoreactivity was barely detected in DN (arrow) and GC (double arrow) of TSC lesions. (D) Statistical analysis results showing that the average OD value of α-syn was reduced in neuropils of both FCD IIb and TSC lesions (***p < 0.001, unpaired two-tailed t-test, n = 7, 9, 8 specimens for each group). (E) Representative images showing α-syn was expressed in vimentin-positive BC in FCD IIb lesions (arrows). (F) α-syn was barely expressed in VGLUT2-positive excitatory neurons in CTX. (G) Representative image showing the co-expression of α-syn and VGLUT2 was intensified in the neuropils, DN (double arrow), and BC (arrow) in FCD IIb lesions. (H) The co-expression of α-syn and VGLUT2 was intensified in the neuropils of TSC lesions as well but not observed in DN and BC. (I) α-syn was not detected in vimentin-positive GCs in TSC lesions. (J) Representative image showing α-syn was barely expressed in VGAT-positive neurons in CTX. (K) Representative image showing significant co-expression of α-syn and VGAT in DN (double arrow) and BC (arrow) of FCD IIb lesions. (L) The co-expression of α-syn and VGAT was intensified in the neuropils of TSC lesions but not observed in DN and GC of TSC lesions. (M) Representative image showing slightly α-syn expression in a NeuN-positive neuron and nearby neuropils in FCD IIb lesions (arrow). (N–P) Representative images showing α-syn was not detected in GFAP-positive astrocytes ((N), arrows), Iba1-positive microglia (O, arrow), and Olig2-positive oligodendrocytes ((P), arrows) in FCD IIb lesions. (Q–T) Representative images showing α-syn was not expressed in NeuN-positive neurons in TSC lesions ((Q), arrow), GFAP-positive ((R), arrows), Iba1-positive microglia ((S), arrows), and Olig2-positive oligodendrocytes in TSC lesions ((T), arrow). Scale bars: 50 μm for the upper panel of (A–C), 20 μm for the bottom panel of (A–C), and 10 μm for images (E–T).
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