Fig 1: Pyrin drives neutrophil caspase-1-dependent pyroptosis in response to Y. pseudotuberculosis ?yopM infectionIndicated BMMs (A–C) and BMNs (D–I) were primed for 16 h, then left non-infected (NI) or infected with either Yptb-WT or ?yopM at MOI 30 for 3 h. Cytotoxicity was determined by LDH release assay (A, D, and G), IL-1ß release was determined by ELISA (B, E, and H), and immunoblots were probed with the indicated antibodies (C, F, and I). Actin was used as a loading control. Data are mean ± SEM of three independent experiments. Cytotoxicity and IL-1ß levels were analyzed by two-way ANOVA followed by Bonferroni’s multiple comparison test (A, B, D, E, G, and H). **p = 0.01, ***p = 0.001, ****p = 0.0001, ns, not significant. Asterisk indicates a non-specific band (I).
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