Fig 1: Phenotypic characterization of AMSCs and GMSCs. (A) CD34, CD166, CD105, CD31 and vWF expression levels in the freshly isolated AMSCs and GMSCs. (B) vWF expression of AMSCs and GMSCs after culture with VEGF (50 ng/ml) for 5 days. CD31 expression of AMSCs and GMSCs after culture with ECs-conditioned medium for 5 days. CD31 and vWF expression levels of AMSCs and GMSCs after co-culture with mature rabbit ECs for 5 days. ECs, endothelial cells; MSCs, mesenchymal stem cells; AMSCs, adipose MSCs; GMSCs, gingival MSCs; vWF, von Willebrand factor.
Fig 2: Culture, isolation and identification of ADSCs. (A), cell morphologies under microscope after isolated cells being culture for respectively 24 h (left) and 14 days (right); (B), detection of biomarkers of ADSCs, CD44 (left) and CD105 (right) using immunofluorescent staining; (C), FCM measured the expressions of CD105, CD166, CD44, CD116, CD34, and CD45 in the 4th generation of ADSCs; (D), osteogenesis differentiation potential of ADSCs was verified using alizarin red staining; (E), adipogenic differentiation potential of ADSCs was verified using oil red O staining; ADSCs, adipose-derived stem cells; FCM, flow cytometry
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