Anti-AKT3 (phospho S472) + AKT2 (phospho S474) + AKT1 (phospho S473) antibody [EPR18853] ab192623 from Abcam

Product Specs
ItemAnti-AKT3 (phospho S472) + AKT2 (phospho S474) + AKT1 (phospho S473) antibody [EPR18853]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab192623
Quantity10 UL, 40 UL, 100 UL
ApplicationsDot Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot, Dot Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot, Dot Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western blot
Conjugate/TagUnconjugated
HostRabbit
Antibody TypePrimary
NCBI Gene AliasesMPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2
ClonalityMonoclonal
ModificationsPhospho-S474
CloneEPR18853
Gene NameAKT3
ReactivityHuman, Mouse, Rat
TargetAKT3+AKT2+AKT1

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Website: http://www.abcam.com

(1) Grape Seed Proanthocyanidin Ameliorates LPS-induced Acute Lung Injury By Modulating M2a Macrophage Polarization Via the TREM2/PI3K/Akt PathwayInflammationDecember 1, 2023Xin Qiao, Hua Wang, Yulin He, Dongfang Song, Abdullah Altawil, Qiuyue Wang, Yan Yin(ab245227) at 1:150 (Abcam, Cambridge, UK), p-PI3K (ab182651) at 1:150 (Abcam, Cambridge, UK), and p-Akt (ab192623) at 1:100 (Abcam, Cambridge, UK) overnight at 4 ◦C. The next day, the lung tissue sections were washed

(2) FAP promotes clear cell renal cell carcinoma progression via activating the PI3K/AKT/mTOR signaling pathwayCancer Cell InternationalSeptember 27, 2023Kun Jiang, Li-zhe Xu, Jin-zhuo Ning, Fan Chengprimary antibodies against FAP (ab207178), PI3K (ab191606), p-PI3K (ab182651), AKT (ab179463), p-AKT (ab192623), mTOR (ab134903), p-mTOR (ab137133) and GADPH (ab181602), which were purchased from Abcam (Cambridge,

(3) The protective mechanism of Dehydromiltirone in diabetic kidney disease is revealed through network pharmacology and experimental validationFrontiers in PharmacologyAugust 23, 2023Yanzhe Wang, Yuyuan Liu, Sijia Chen, Fengqin Li, Yue Wu, Xinmiao Xie, Nan Zhang, Chuchu Zeng, Linnan Bai, Mengshi Dai, et al.ab40776), PI3K (1:1000, ab191606), p-PI3K (1:1000, ab182651), AKT (1:1000, ab179463), and p-AKT (1:1000, ab192623) were bought from Abcam Co. (Cambridge, United Kingdom). Goat Anti-Rabbit IgG H&L (HRP, A0208), Rabbit

(4) The Synergistic Effect of Zuogui Pill and Eldecalcitol on Improving Bone Mass and Osteogenesis in Type 2 Diabetic OsteoporosisMedicina (Kaunas)August 3, 2023Tuo Shi, Ting Liu, Yuying Kou, Xing Rong, Lingxiao Meng, Yajun Cui, Ruihan Gao, Sumin Hu, Minqi Li(Beijing, China). The anti-alkaline phosphatase (ALP) (ab65834), anti-P-PI3K (ab278545), and anti-P-AKT (ab192623) antibodies as well as goat anti-rabbit (ab6721), goat anti-mouse (SA00001-1), and goat anti-rabbit

(5) An iRGD-conjugated photothermal therapy-responsive gold nanoparticle system carrying siCDK7 induces necroptosis and immunotherapeutic responses in lung adenocarcinomaBioengineering & translational medicineJuly 1, 2023Cai R, Wang M, Liu M, Zhu X, Feng L, Yu Z, Yang X, Zhang Z, Guo H, Guo R, et al.5 min with PBST and incubated with primary antibodies against Akt (Abcam, ab179463), p‐AKT (Abcam, ab192623), STAT3 (Abcam, ab68153), p‐STAT (Abcam, ab76315), GAPDH (Proteintech, 60004‐1‐Ig), CDK7 (Abcam, ab256787),

(6) Isorhamnetin attenuates the proliferation, invasion, migration and fibrosis of keloid fibroblasts by targeting S1PR1Experimental and therapeutic medicineJuly 1, 2023Pu X, Cao X, Liu H, Huang W, Zhang L, Jiang T.55133-1-AP, Proteintech), p-PI3K (1:1,000, ab191606, Abcam), PI3K (1:1,000, ab182651, Abcam), p-AKT (1:1,000, ab192623, Abcam), AKT (1:1,000, ab179463, Abcam), and GAPDH (1:2,500, ab9485, Abcam). Next, the membranes were

(7) Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal pigletsPediatric researchJune 1, 2023Suryawan A, Rudar M, Naberhuis JK, Fiorotto ML, Davis TA.non-phospho-antibodies. The primary antibodies that were used were: phospho-pan-/Akt (Ser472/473/474) (1:1000; #ab192623; Abcam, Cambridge, MA); pan-Akt, Akt1, Akt2, Akt3 (Rabbit mAb #4685, Rabbit mAb #75692, Rabbit mAb #3063,

(8) Kaixin Jieyu Granule attenuates neuroinflammation-induced depressive-like behavior through TLR4/PI3K/AKT/FOXO1 pathway: a study of network pharmacology and experimental validationBMC Complementary Medicine and TherapiesMay 12, 2023Manman Xu, Wujianwen Zhai, Ying Zhang, Juhua Pan, Jie Li, Shijing Huangwere purchased from BioProducts MD (Middletown, MD, USA). The antibodies to PI3K (ab178846), p-AKT (ab192623), AKT (ab179463), p-PTEN (ab76431), PTEN (ab170941), p-FOXO1 (ab131339), and FOXO1 (ab52857) were from

(9) LonP1 Drives Proneural Mesenchymal Transition in IDH1-R132H Diffuse GliomabioRxiv : the preprint server for biologyApril 17, 2023Douglas C, Lomeli N, Vu T, Pham J, Bota DA.(Fisher Scientific, PA5-80107) 1:2000 B-Actin (Novus Biologicals, NB600-501), 1:1000 p-AKT (Abcam, ab192623-100ul), 1:10,000 goat anti-mouse IgG F(ab’)2 (Enzo Life Sciences, ADI-SAB-100-J), and 1:3,000 IgG (H+L)

(10) Discovery and Validation of Novel LonP1 and Proteasome Inhibitor in IDH1-R132H Malignant Astrocytoma ModelsbioRxiv : the preprint server for biologyApril 17, 2023Douglas C, Lomeli N, Lepe J, Di K, Nandwana NK, Vu T, Pham J, Kenney MC, Das B, Bota DA.(Fisher Scientific, PA5–80107) 1:2000 B-Actin (Novus Biologicals, NB600–501), 1:1000 p-AKT (Abcam, ab192623–100ul), 1:10,000 goat anti-mouse IgG F(ab’)2 (Enzo Life Sciences, ADI-SAB-100-J), and 1:3,000 IgG (H+L)

(11) Psoralidin inhibits osteosarcoma growth and metastasis by downregulating ITGB1 expression via the FAK and PI3K/Akt signaling pathwaysChinese MedicineMarch 31, 2023Shengwen Cheng, Senrui Liu, Bowen Chen, Chengcheng Du, Pengcheng Xiao, Xuefeng Luo, Li Wei, Yiting Lei, Chen Zhao, Wei Huang(phospho Y607) (ab182651, Abcam), anti-Akt (ab179463, Abcam), and anti-p-Akt (phospho S472 + S473 + S474) (ab192623, Abcam). RNA-sequencing 143B and MG63 cells were treated with 30 μM PSO for 24 h. Total RNA was extracted

(12) Targeting IGF1R signaling enhances the sensitivity of cisplatin by inhibiting proline and arginine metabolism in oesophageal squamous cell carcinoma under hypoxiaJournal of Experimental & Clinical Cancer ResearchMarch 28, 2023Kang Fang, Mingchuang Sun, Zhuyun Leng, Yuan Chu, Ziying Zhao, Zhaoxing Li, Yunwei Zhang, Aiping Xu, Zehua Zhang, Li Zhang, et al.(ab138005), STAT3 (ab32500), STAT3-P (ab267373), PI3K (ab191606), PI3K-P (ab182651), AKT (ab179463), AKT-P (ab192623), mTORC (ab134903), and β-actin (ab8227) were purchased from Abcam (Cambridge, UK). Immunoblotting assays

(13) CDCA4 interacts with IGF2BP1 to regulate lung adenocarcinoma proliferation via the PI3K/AKT pathwayThoracic cancerMarch 1, 2023Feng S, Cao H, Sui Y, Shen Z, Wu J, Ma R, Feng J.(ab267787, Abcam), PI3K (ab191606, Abcam), P‐PI3K (ab182651, Abcam), AKT (ab179463, Abcam), P‐AKT (ab192623, Abcam), CDK2 (10122‐1‐AP, Proteintech), and cyclinA2 (18202‐1‐AP, Proteintech) overnight at 4°C. The

(14) Platycodin D inhibits the proliferation, invasion and migration of endometrial cancer cells by blocking the PI3K/Akt signaling pathway via ADRA2A upregulationOncology LettersFebruary 15, 2023Zhen Ni, Zhuoma Dawa, Deji Suolang, Quzhen Pingcuo, Zhuoma Langga, Pingcuo Quzhen, Zhuoga Deji(1:1,000; ab76003), ADRA2A (1:1,000; ab85570), phospho (p)-PI3K (1:1,000; ab182651), p-Akt (1:1,000; ab192623), PI3K (1:1,000; ab191606), Akt (1:1,000; ab179463) and GAPDH (1:2,500; ab9485), all from Abcam. After

(15) Feiyiliu Mixture sensitizes EGFRDel19/T790M/C797S mutant non-small cell lung cancer to osimertinib by attenuating the PRC1/Wnt/EGFR pathwayFrontiers in PharmacologyJanuary 19, 2023Jingjing Shi, Shaoyu Hao, Xiantao Liu, Yingying Li, Xin ZhengChina, 1:1000), c-Jun (#9165, Cell Signaling Technology, 1:1000), Akt (ab179463, Abcam, 1:10000), p-Akt (ab192623, Abcam, 1:1000), β-actin (BA2305, BOSTER, Wuhan, China, 1:5000), GAPDH (A00227-1, BOSTER, Wuhan, China,

(16) SARS-CoV-2 replication in airway epithelia requires motile cilia and microvillar reprogrammingCellJanuary 5, 2023Wu CT, Lidsky PV, Xiao Y, Cheng R, Lee IT, Nakayama T, Jiang S, He W, Demeter J, Knight MG, et al.RRID:AB_2158623 Rabbit anti-PAK4,Cell Signaling,Cat# 62690S RRID:AB_2827508 Rabbit anti-Phospho-AKT,Abcam,Cat# ab192623 Rabbit anti- Phospho-Erk1/2,Cell Signaling,Cat# 9101S RRID:AB_331646 Mouse anti- Phospho-Erk1/2,Cell Signaling

(17) RNF7 promotes glioma growth via the PI3K / AKT signalling axisJournal of Cellular and Molecular MedicineJanuary 1, 2023Nan Tang, Kai Zhu, Cheng Jiang, Zhiyong Xiong, Qiangping Wang, Junjun Li, Weiming XuLouis, MO). Primary antibodies were used to detect RNF7 (ab181986; Abcam), AKT (ab179463; Abcam), p‐AKT (ab192623; Abcam), PI3K (ab191606; Abcam) and p‐PI3K (ab182651; Abcam). For more detailed information, please

(18) Metformin Improves Burn Wound Healing by Modulating Microenvironmental Fibroblasts and MacrophagesCellsDecember 16, 2022Shi L, Jiang Z, Li J, Lin H, Xu B, Liao X, Fu Z, Ao H, Guo G, Liu M.antibody (Abcam, ab179463, 1:10000);Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474) antibody (Abcam, ab192623, 1:1000);Anti-MMP2 antibody (Abcam, ab181286, 1:1000);Anti-MMP9 antibody (Abcam, ab228402, 1:1000);Anti-NF-κB

(19) Scabertopin Derived from Elephantopus scaber L. Mediates Necroptosis by Inducing Reactive Oxygen Species Production in Bladder Cancer In VitroCancers (Basel)December 2, 2022Yuanhui Gao, Zhenyu Nie, Hui Cao, Denggao Huang, Mei Chen, Yang Xiang, Xiaolong Yu, Shufang ZhangBcl-2-associated X (Bax, ab32503), phospho-PI3K (Y607, ab182651), AKT (ab179463), phospho-AKT (S472, S473, S474, ab192623), and GAPDH (ab181602) antibodies were purchased from Abcam (Cambridge, MA, USA). Gasdermin D (97558),

(20) The role of AURKA / miR ‐199b‐3p in hepatocellular carcinoma cellsJournal of Clinical Laboratory AnalysisDecember 1, 2022Guogang Li, Yang Tian, Zhenzhen GaoAbcam), anti‐protein kinase B (AKT) (1:10,000, ab179463), anti‐phosphorylated‐AKT (p‐AKT) (1:1000, ab192623, Abcam), and rabbit anti‐GAPDH (1:10,000, ab8245, Abcam) at 4°C overnight. Subsequently, the membrane

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Anti-AKT3 (phospho S472) + AKT2 (phospho S474) + AKT1 (phospho S473) antibody [EPR18853] from Abcam

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Anti-AKT3 (phospho S472) + AKT2 (phospho S474) + AKT1 (phospho S473) antibody [EPR18853] from Abcam

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