Fig 2: miR-6745 suppresses gastric tumor growth in vivo. Subcutaneous xenografts of GC cells infected with miR-6745 overexpressing lentivirus (Lv-miR-6745) or control lentivirus (Lv-miR-NC). (A) Images of the tumors at autopsy from nude mice are presented. (B) Tumor volumes and average weight of xenografted tumors were measured. (C) Immunohistochemical (IHC) staining of TIMP1 and Ki67 in xenografted tumors from Lv-miR-6745 cells or control cells. Data represent the means ± SEM. **P < 0.01.

Fig 3: Protein and mRNA expression levels of CDH2, LEP, POSTN, TIMP1 and VEGFC in colon cancer cells following transfection with target siRNAs. After silencing of (A) CDH2, (B) LEP, (C) POSTN, (D) TIMP1 and (E) VEGFC in the colon cancer cells using siRNAs, the mRNA and protein expression levels were detected using reverse transcription-quantitative PCR and western blot analysis. The data was analyzed using an unpaired Student's t-test and expressed as the mean ± SD. Each assay was performed in independently 3 times. **P<0.01. ***P<0.001. si, small interfering.

Fig 4: The effects of kindlin-3 silencing on cell proliferation, invasion, and epithelial–mesenchymal transition (EMT) in AML cells. HL-60 and Kg1a cells were transfected with si-kindlin-3 or si-NC for 48 h. (A) The protein expressions of kindlin-3 were determined by Western blot assay. (B) Cell proliferation was assessed by BrdU-ELISA assay. (C) The mRNA expressions of PCNA, CDK4, cyclin D1, and p27 were determined by qRT-PCR. (D) The invasion was assessed by Transwell assay. (E) The protein expressions of MMP-2, MMP-9, and TIMP-1 were detected by Western blot assay. (F) Cell apoptosis was measured by nucleosomal degradation by using Roche’s cell death ELISA detection kit. (G) The activities of caspase 3 were determined by caspase 3 activity detection assay. All data are presented as mean ± SEM, n = 6. #p < 0.05, ##p < 0.01 versus si-NC.

Fig 5: TIMP-1 (A,B) and collagen a1 (I) (C,D) immunohistochemical analysis of a 4-day-old wound (A,B,D) with adjacent skin (C). (A) Fibroblasts (solid arrowheads) below neoepidermis and (B) endothelial cell (outline arrowhead) of small blood vessel show positive immunoreactivity of TIMP-1 in upper dermis. (D) Arrow indicates the tip of the migrating neoepidermal tongue.