Fig 1: The mucus in usual interstitial pneumonia has distinct features from mucinous adenocarcinoma. A Venn diagram showing the proteins detected in the mucus of mucinous adenocarcinoma [MA] (n = 6 patients) or usual interstitial pneumonia (UIP; n = 6 patients). B 3-dimensional principal component analysis for each mucus type. C Volcano plot comparing the UIP mucus to mucinous adenocarcinoma mucus showing the negative natural log of the false discovery values (FDR) values plotted against the base 2 log (fold change) for each protein. D Immunofluorescence for MUC5B, MUC5AC, and BPIFB1 in UIP mucus (n = 4 patients) and mucinous adenocarcinoma mucus (n = 3 patients) with representative images shown for each disease type. White arrows points to regions of mucus accumulation and asterisk shows positivity of MUC5AC within UIP mucus. Scale bar represents 100 microns
Fig 2: The composition of fibrotic mucus. Laser capture microdissection coupled mass spectrometry was performed on the mucus plugs of 6 Usual Interstitial Pneumonia (UIP) patients. A A list of the most abundant secretome-associated proteins found in the fibrotic mucus shown as intensity Based Absolute Quantification (iBAQ). B Reactome pathway enrichment of UIP mucus represented as a dotplot. C Serial sections of UIP specimens stained for alcian blue/periodic acid Schiff’s (AB/PAS) or immunostained for MUC5B, MUC5AC, BPIFB1, PIGR, and TF (N = 4 UIP patients with 2 representative images shown). Scale bar represents 100 microns
Supplier Page from Abcam for Anti-BPIFB1 antibody [2A5]