Fig 1: GECs express Rev-erba during H pylori infection. (A) Representative immunofluorescence staining images showing Rev-erba expression (red) in cytoplasm and nucleus (arrow) of CD326+ cells (green) and Rev-erba–expressing (red) pepsinogen II+ chief cells (green) in gastric corpus of H pylori–infected patients. Scale bars: 10 µm or 100 µm. (B) Representative immunofluorescence staining images showing Rev-erba–expressing (red) cells and H pylori (green) colonization in gastric mucosa of H pylori–infected patients. Scale bars: 10 µm. (C) Representative immunofluorescence staining images showing Rev-erba expression (red) in WT H pylori–infected, and uninfected AGS cells (MOI = 100, 6 hours). Scale bars: 1 µm. (D) Rev-erba expression in the nucleus of WT H pylori–infected, and uninfected AGS cells (MOI = 100, 24 hours) was analyzed by Western blot. The expression of cagA protein in AGS cells infected with WT H pylori or ?cagA (MOI = 100, 24 hours) was analyzed by Western blot. (E, F) Rev-erba mRNA expression and Rev-erba protein in WT H pylori–infected and (E) uninfected AGS cells or (F) human primary GECs at different time point (MOI = 100) or with different MOI (24 hours) were analyzed by real-time PCR or Western blot (n = 3). (G) Rev-erba mRNA expression and Rev-erba protein in WT H pylori–infected, ?cagA-infected, and uninfected AGS cells or human primary GECs (MOI = 100, 24 hours) were analyzed by real-time PCR and Western blot (n = 3). (H) Rev-erba expression in WT H pylori–infected, ?cagA-infected, and uninfected GES-1 cells, HGC-27 cells, SGC-7901 cells, and BGC-823 cells (MOI = 100, 24 hours) was analyzed by real-time PCR and Western blot (n = 3). *P < .05, **P < .01 for groups connected by horizontal lines.