Fig 1: Expression and localization of TSLPR-expressing cells in the BM specimens.A, Representative images of paraffin sections stained with CD11c and CD68 antibodies (brown). Counterstaining with Mayer’s hematoxylin was subsequently performed (blue). Scale bars, 100 µm. B, Double immunofluorescence staining performed with anti-CD11c (green) or anti-CD68 (green), anti-TSLPR (red), and DAPI for staining nuclei (blue). White arrows indicate co-localizing staining of cells (yellow). Scale bars, 100 µm. C, The number and ratio of merged cells to TSLPR+ cells from 15 patients with OLP calculated by double immunofluorescence staining. Statistical significance of differences between groups was determined by Mann—Whitney U tests (*P < 0.05).
Fig 2: Correlation between the number of CD11c+ and GATA3+ cells in BM specimens.The number of these positive cells in BM samples from patients with oral lichen planus (OLP) (n = 15), hyperkeratosis (n = 5) and ulcer (n = 5) was calculated from immunohistochemical staining as described in the Methods section. Statistical significance of differences between groups was determined by Spearman’s rank correlation (p < 0.05).
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