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PE Mouse IgG2b, kappa isotype Ctrl
PE Mouse IgG2b, kappa isotype Ctrl from BioLegend
(2)
Write a review
Citations:
(46)
Supplier Page
Supplier Page from
BioLegend for
PE Mouse IgG2b, kappa isotype Ctrl
BioLegend's
PE Mouse IgG2b, kappa isotype Ctrl
is a Mouse monoclonal antibody. This antibody has been shown to work in applications such as: Staining, and Flow Cytometry.
Description
PE Mouse IgG2b, kappa isotype Ctrl [MPC-11]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
PE Mouse IgG2b, kappa isotype Ctrl
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
400314
Quantity
100 tests
Applications
Flow Cytometry (FC), Intracellular Staining for Flow Cytometry (ICFC)
Conjugate/Tag
PE
Host
Mouse
Isotype
IgG2b, Kappa
Clone
MPC-11
Format
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Clonality
Monoclonal
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (46)
(1)
Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaques
Nature biotechnology
March 1, 2024
Hu X, White K, Olroyd AG, DeJesus R, Dominguez AA, Dowdle WE, Friera AM, Young C, Wells F, Chu EY,
et al
.
BioLegend
), anti-PD-L1 (PE,
clone
29E.2A3,
329705
,
1:20
dilution,
BioLegend
with IgG2b, PE,
clone
MPC-11,
400313
,
1:20
dilution,
BioLegend
) or anti-CD47 (FITC,
clone
CC2C6,
323106
,
1:20
dilution,
BioLegend
with IgG1,
(2)
Combination of irinotecan silicasome nanoparticles with radiation therapy sensitizes immunotherapy by modulating the activation of the cGAS/STING pathway for colorectal cancer
Materials Today Bio
December 1, 2023
Lu Wang, Tianyu Zhang, Yile Zheng, Yuting Li, Xiyuan Tang, Qianping Chen, Wei Mao, Weiwei Li, Xiangsheng Liu, Ji Zhu
antibodies for 30 min on ice. The IgG group was incubated with an equal amount of isotype IgG (#
400219
; #
400313
;
BioLegend
). Cells were twice washed with PBS and were then detected by
flow cytometry
. 4.9 Reverse
(3)
Multicolor flow cytometry-based immunophenotyping for preclinical characterization of nanotechnology-based formulations: an insight into structure activity relationship and nanoparticle biocompatibility profiles
Frontiers in Allergy
July 4, 2023
Hannah S. Newton, Jenny Zhang, Duncan Donohue, Ragi Unnithan, Edward Cedrone, Jie Xu, Alison Vermilya, Tyler Malys, Jeffrey D. Clogston, Marina A. Dobrovolskaia
κ",MOPC-21,
BioLegend
,200,
400109
or
400110
,"1, 2" PE,"Mouse IgG2b, κ",MPC-11,
BioLegend
,200,
400313
or
400314
,1 PE,"Mouse IgG2a, κ",MOPC-173,
BioLegend
,200,
400211
,2 PE-Cy7,"Mouse IgG1, κ",MOPC-21,
BioLegend
,200,
400125
(4)
Mitotic bookmarking by SWI/SNF subunits
Nature
June 1, 2023
Zhu Z, Chen X, Guo A, Manzano T, Walsh PJ, Wills KM, Halliburton R, Radko-Juettner S, Carter RD, Partridge JF,
et al
.
PE anti-Histone H3 Phospho (Ser10
) Antibody (
BioLegend
#
650808
) or PE Mouse IgG2b, κ Isotype Ctrl Antibody (
BioLegend
#
400314
) and incubated for 20 min in the dark at room temperature. Next, cells were washed twice with 2 ml of
(5)
A Modified Method for the Quantification of Immune Checkpoint Ligands on Exosomes from Human Serum using Flow Cytometry
Technol Cancer Res Treat
May 15, 2023
Alan Guillermo Alejandre Gonzalez, Pablo Cesar Ortiz-Lazareno, Fabiola Solorzano-Ibarra, Jorge Gutierrez-Franco, Martha Cecilia Tellez-Bañuelos, Miriam Ruth Bueno-Topete, Susana Del Toro-Arreola, Jesse Haramati
and the following isotype controls were also used: APC (Cat.
400120
, Lot 243040,
Biolegend
), PE (Cat.
400314
, Lot B229091, Biolegend), and PE-Cy7 (Cat.
400126
, Lot B308653
Biolegend
). After the addition of the
(6)
Repurposing pentamidine for cancer immunotherapy by targeting the PD1/PD-L1 immune checkpoint
Frontiers in Immunology
May 2, 2023
Tingxuan Gu, Xueli Tian, Yuanyuan Wang, Wenqian Yang, Wenwen Li, Mengqiu Song, Ran Zhao, Mengqiao Wang, Quanli Gao, Tiepeng Li,
et al.
were employed to stain
human
and murine
PD-L1
in
flow cytometry
sorting. Isotype PE mouse IgG2b (Cat#
400313
,
Biolegend
) and APC rat IgG2b (Cat#
400612
,
Biolegend
) were used as isotype control antibodies. 2.13
(7)
Influence of chemoradiation on the immune microenvironment of cervical cancer patients
Strahlentherapie und Onkologie
February 1, 2023
J. M. Herter, M. Kiljan, S. Kunze, M. Reinscheid, O. Ibruli, J. Cai, L. Niu, I. Heßelmann, M. Trommer, G. S. Herter-Sprie,
et al.
purchased from
Biolegend
: CD16-FITC (#
302006
),
CD19
-PerPC-Cy5.5 (#
302230
),
PD-L1
-PE (#
329706
), IgG2b-PE (#
400314
),
CD56
-PE-Dazzle 594 (#
362544
), MHC-I-PE-Cy7 (#
311430
),
CD66b
-APC (#
305118
),
CD45
-Alexa700 (#
368514
),
(8)
Soluble PD-L1: a potential dynamic predictive biomarker for immunotherapy in patients with proficient mismatch repair colorectal cancer
Journal of Translational Medicine
January 13, 2023
Yinjun He, Xiang Zhang, Ming Zhu, Wenguang He, Hanju Hua, Feng Ye, Xile Zhou, Nan Chen, Yandong Li, Weixiang Zhong,
et al.
of the detection antibody anti-PD-L1 PE (
329706
,
Biolegend
) or the labeled isotype control antibody (
400314
,
Biolegend
) for 1 h at room temperature. The complexes were resuspended in 100 µL PBS after washing
(9)
Ribonuclease 1 Enhances Antitumor Immunity against Breast Cancer by Boosting T cell Activation
International journal of biological sciences
January 1, 2023
Wang YN, Lee HH, Jiang Z, Chan LC, Hortobagyi GN, Yu D, Hung MC.
BioLegend
, #
400125
), APC mouse IgG2a (
1:200
;
BioLegend
, #
400219
), PE mouse IgG2b (
1:200
;
BioLegend
, #
400313
), and FITC mouse IgG1 (
1:200
;
BioLegend
, #
400104
) as control
staining
, respectively. For analyzing
EphA4
(10)
Comprehensive Evaluation of Multiple Approaches Targeting ABCB1 to Resensitize Docetaxel-Resistant Prostate Cancer Cell Lines
International Journal of Molecular Sciences
December 30, 2022
Dinah Linke, Lukas Donix, Claudia Peitzsch, Holger H. H. Erb, Anna Dubrovska, Manuel Pfeifer, Christian Thomas, Susanne Fuessel, Kati Erdmann
clone
5D3, 5 µg/mL;
Biolegend
) or corresponding isotype controls (IgG2a:
400214
,
clone
MOPC-173, IgG2b:
400314
,
clone
MPC-11;
Biolegend
) conjugated with phycoerythrin (PE). After
incubation
for 30 min at 4 °C in
(11)
Highly sensitive and non-disruptive detection of residual undifferentiated cells by measuring miRNAs in culture supernatant
Scientific Reports
December 1, 2022
Kanako Masumoto, Yuki Aihara, Mao Miyagawa Kuroishi, Natsuki Maeda, Yumiko Sakai, Yuma Oka, Yusuke Takahashi, Kenta Oda, Masatoshi Yanagida
number of
Oct4
-positive cells was determined using Amnis™ ImageStream™ X MkII (
Luminex
). PE Mouse IgG2b (
400314
,
BioLegend
) was used to set the gate. Hoechst stain was used to stain the nucleus. After seeding iPSCs
(12)
In mouse chronic pancreatitis CD25(+)FOXP3(+) regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response
Nature communications
August 3, 2022
Glaubitz J, Wilden A, Golchert J, Homuth G, Völker U, Bröker BM, Thiele T, Lerch MM, Mayerle J, Aghdassi AA,
et al
.
IgG1, Isotype Ctrl (dilution
1:50
,
400417
,
BioLegend
), PE Mouse IgG2b, Isotype Ctrl (dilution
1:50
,
400313
,
BioLegend
), PE Mouse IgG1, Isotype Ctrl (dilution
1:50
,
551436
,
BD
), APC Rat IgG2b, Isotype Ctrl (dilution
(13)
Galectin-9 contributes to the pathogenesis of atopic dermatitis via T cell immunoglobulin mucin-3
Frontiers in Immunology
July 22, 2022
Wenxing Su, Ji Zhang, Shun Yang, Minhui Tang, Yu Shen, Cuiping Liu, Jiang Ji, Marcus Maurer, Qingqing Jiao
(
400108
,
Clone
: RTK2071,
Biolegend
), mouse IgG2a (
400246
,
Clone
: MOPC-173, Biolegend), and mouse IgG 2b (
400314
,
Clone
: MPC-11,
Biolegend
) were used as isotype controls. Cells were analyzed with a Coulter Epics XL
(14)
A New Method of Bone Stromal Cell Characterization by Flow Cytometry
Current Protocols
March 29, 2022
Chirayu Patel, Lihong Shi, John F. Whitesides, Brittni M. Foster, Roberto J. Fajardo, Ellen E. Quillen, Bethany A. Kerr
OSTEOCLAST
,
MACROPHAGE
/
OSTEOCLAST
,
MACROPHAGE
/
OSTEOCLAST
,
MACROPHAGE
/
OSTEOCLAST
"PE Mouse IgG2b, κ",
BioLegend
,
400314
,N.A "FITC Rat IgG2b, κ",
BioLegend
,
400634
,AB_893662 "PerCP-Cy5.5 Mouse IgG 2b,k",
BioLegend
,
400338
,N.A. "APC
(15)
Genetic analysis of cancer drivers reveals cohesin and CTCF as suppressors of PD-L1
Proceedings of the National Academy of Sciences of the United States of America
February 15, 2022
Oreskovic E, Wheeler EC, Mengwasser KE, Fujimura E, Martin TD, Tothova Z, Elledge SJ.
(
BioLegend
catalog no.
311404
). Isotype controls used were mouse IgG2b, k-PE (
BioLegend
catalog no.
400313
), and mouse IgG2a, k-FITC (
BioLegend
catalog no.
400208
). Cells were washed twice after
staining
and
(16)
Development of a Stable Peptide-Based PET Tracer for Detecting CD133-Expressing Cancer Cells
ACS Omega
January 11, 2022
Kuan Hu et al.
CD133 Antibody (Rat IgG2a, λ, cat.
141204
, CA, USA), PE Mouse IgG2b (κ Isotype Ctrl Antibody, cat.
400314
,
BioLegend
, CA, USA), and
PE anti-human IgG Fc
Antibody (Rat IgG2a, κ, cat. 410708) were used
for flow cytometry
(17)
SOX10 requirement for melanoma tumor growth is due, in part, to immune-mediated effects
Cell reports
December 7, 2021
Rosenbaum SR, Tiago M, Caksa S, Capparelli C, Purwin TJ, Kumar G, Glasheen M, Pomante D, Kotas D, Chervoneva I,
et al
.
control",BioLegend,MOPC-21; RRID:AB_893664 "Mouse IgG2b, κ isotype control",
BioLegend
,MPC-11; Catalog no.
400313
CD8α,BioXCell,53-6.72; RRID:AB_1107671 Rat IgG2a,BioXCell,2A3; RRID:AB_1107769 ,, "Chemicals, peptides,
(18)
Transcriptional Reprogramming and Constitutive PD-L1 Expression in Melanoma Are Associated with Dedifferentiation and Activation of Interferon and Tumour Necrosis Factor Signalling Pathways
Cancers (Basel)
August 24, 2021
Antonio Ahn, Euan J. Rodger, Jyoti Motwani, Gregory Gimenez, Peter A. Stockwell, Matthew Parry, Peter Hersey, Aniruddha Chatterjee, Michael R. Eccles
(
Biolegend
, catalog#
329706
) antibody and the isotype control antibody (PE Mouse IgG2b,
Biolegend
, catalog#:
400314
) have a maximum excitation at 575 nm. No overlap in
fluorescence
emission was detected between the FVS450
(19)
KPNB1 Inhibitor Importazole Reduces Ionizing Radiation-Increased Cell Surface PD-L1 Expression by Modulating Expression and Nuclear Import of IRF1
Current Issues in Molecular Biology
May 19, 2021
Hironori Yoshino, Yoshiaki Sato, Manabu Nakano
(PE)-labeled anti-human PD-L1 monoclonal antibody (#
329706
) and PE-conjugated anti-mouse IgG2b antibody (#
400314
) were purchased from
BioLegend
(San Diego, CA, USA). Ambion Silencer® Select Pre-designed small interfering
(20)
Inflamed Ulcerative Colitis Regions Associated With MRGPRX2-Mediated Mast Cell Degranulation and Cell Activation Modules, Defining a New Therapeutic Target
Gastroenterology
April 1, 2021
Ernie Chen et al.
stained with PE anti-human MRGX2 Antibody (
359003
;
BioLegend
) or PE Mouse IgG2b k Isotype Control (
400314
;
BioLegend
) at a concentration of 1.25 ul of antibody per 1 million cells in a final volume of 100 ul
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Reviews
Write a Review
(1)
1/30/2019
Staining Cultured MSC with Mouse IgG2b PE Isotype Control
Adam Guess
This antibody was used as an isotype control to account for non-specific background staining in cultured MSC. It was chosen because it was the recommended isotype control for an antibody in a ...
Read Review
(1)
2/9/2016
Excellent PE Isotype Mouse IgG2b
Linda Hammerich
This antibody was used as an isotype control in intracellular flow cytometry and compared to anti-HMGB1-PE to determine the specifity of the HMGB-1 antibody.
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<
>
>>
PE Mouse IgG2b, kappa isotype Ctrl from BioLegend
Supplier Page
View Company Product Page
Pricing Info
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Company Info
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