APC Mouse IgG2a, kappa isotype Ctrl (FC) 400222 from BioLegend

Product Specs
ItemAPC Mouse IgG2a, kappa isotype Ctrl (FC)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number400222
CloneMOPC-173
Quantity100 tests
IsotypeIgG2a, Kappa
Conjugate/TagAPC
ApplicationsFlow Cytometry (FC)
HostMouse
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ClonalityMonoclonal

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Hypoimmune induced pluripotent stem cells survive long term in fully immunocompetent, allogeneic rhesus macaquesNature biotechnologyMarch 1, 2024Hu X, White K, Olroyd AG, DeJesus R, Dominguez AA, Dowdle WE, Friera AM, Young C, Wells F, Chu EY, et al.BioLegend), anti-HLA-G (APC, clone 87G, 335909, 1:20 dilution, BioLegend with IgG2a, APC, clone MOPC-173, 400221 1:20 dilution, BioLegend), anti-PD-L1 (PE, clone 29E.2A3, 329705, 1:20 dilution, BioLegend with IgG2b,

(2) N6-methyladenosine modification promotes hepatocarcinogenesis through circ-CDYL-enriched and EpCAM-positive liver tumor-initiating exosomesiScienceOctober 20, 2023Yanping Wei, Jingbo Fu, Hailing Zhang, Yan Ling, Xuewu Tang, Shuowu Liu, Miao Yu, Fuyan Liu, Guokun Zhuang, Haihua Qian, et al.monoclonal antibodies anti-EpCAM APC (369810, Biolegend, San Diego, CA) or with the isotype control (400222, Biolegend, San Diego, CA) at 4°C in the dark overnight. The samples were analyzed using a Beckman MoFlo XDP

(3) Combined Heterozygous Genetic Variations in Complement C2 and C8B: An Explanation for Multidimensional Immune Imbalance?Journal of Innate ImmunityMarch 1, 2023Marco Mannes, Rebecca Halbgebauer, Lisa Wohlgemuth, David Alexander Christian Christian Messerer, Susa Savukoski, Anke Schultze, Bettina Berger, Christiane Leonie Knapp, Christoph Q. Schmidt, Daniel Fürst, et al.isotype: 400326, BioLegend, USA), anti-CD88 (134 ng/mL, APC-conjugated, 344310, BioLegend, USA; isotype: 400222, BioLegend, USA). Reactive oxygen species production was measured in heparinized blood by using the

(4) Engineering human stem cell-derived islets to evade immune rejection and promote localized immune toleranceCell Reports MedicineJanuary 17, 2023Dario Gerace, Quan Zhou, Jennifer Hyoje-Ryu Kenty, Adrian Veres, Elad Sintov, Xi Wang, Kyle R. Boulanger, Hongfei Li, Douglas A. Melton362519). Isotype controls were Pacific Blue mouse IgG1 (Biolegend, 400131), APC mouse IgG2a (Biolegend, 400221) and PE rat IgG2b (Biolegend, 400607). All antibodies were used at 1:100 or unless otherwise stated.

(5) The regional distribution of resident immune cells shapes distinct immunological environments along the murine epididymiseLifeDecember 14, 2022Pleuger C, Ai D, Hoppe ML, Winter LT, Bohnert D, Karl D, Guenther S, Epelman S, Kantores C, Fijak M, et al.1:200 Isotype control Antibody,"APC Mouse IgG2a, κ Isotype Ctrl (FC) antibody",BioLegend,Cat.No.: 400221; RRID: AB_2891178,FC 1:200 Isotype control Antibody,"APC/Cyanine7 Rat IgG2b, κ Isotype Ctrl antibody",BioLegend,Cat.No.:

(6) The injured sciatic nerve atlas (iSNAT), insights into the cellular and molecular basis of neural tissue degeneration and regenerationeLifeDecember 14, 2022Zhao XF, Huffman LD, Hafner H, Athaiya M, Finneran MC, Kalinski AL, Kohen R, Flynn C, Passino R, Johnson CN, et al.monoclonal),BioLegend,109813,1:100 Antibody,Isotype Control-APC(mouse-IgG2a monoclonal),BioLegend,400221,1:100 Antibody,Ly6G-APC-Cy7(rat-IgG2a monoclonal),BD Biosciences,560600,1:100 Antibody,CD11c-PerCP-Cy5.5(ArmHam-IgG

(7) Characterization of Aldosterone-producing Cell Cluster (APCC) at Single-cell ResolutionThe Journal of Clinical Endocrinology & MetabolismAugust 18, 2022Norifusa Iwahashi et al.determined by comparison with the fluorescence intensity of APC-conjugated isotype IgG (BioLegend Cat# 400221, RRID:AB_2891178), and the cellular regions of specific binding to CD56 antibody and nonspecific binding

(8) Gut microbiota regulation of P-glycoprotein in the intestinal epithelium in maintenance of homeostasisMicrobiomeDecember 1, 2021Sage E. Foley et al.clone (BioLegend catalog no. 348607), or isotype control APC mouse IgG2a(k) (BioLegend catalog no. 400221). Cells were washed and resuspended in 4=, 6-diamidino-2-phenylindole (DAPI; Thermo) for live/dead differentiation.

(9) Intron retention-induced neoantigen load correlates with unfavorable prognosis in multiple myelomaOncogeneOctober 21, 2021Chuanpeng Dong, Annamaria Cesarano, Giuseppe Bombaci, Jill L. Reiter, Christina Y. Yu, Yue Wang, Zhaoyang Jiang, Mohammad Abu Zaid, Kun Huang, Xiongbin Lu, et al.used: HLA-DR, DQ, DP-APC (Biolegend, San Diego, CA cat# 361714), Isotype control-APC (Biolegend cat# 400222), MHC class I-PE (LSBio, Seattle, WA cat# LS-C751033-0.1). Isotype control-PE (Abcam, Waltham, MA cat#

(10) Activating Mucosal-Associated Invariant T Cells Induces a Broad Antitumor ResponseCancer Immunology ResearchSeptember 1, 2021Benjamin Ruf et al.anti-MR1-APC (BioLegend, cat. #361108, RRID:AB_2563204) or isotype control (mouse IgG2a, κ, Biolegend, cat. #400222, RRID:AB_2891178) for surface staining. Targeted knockout of MR1 in B16F10-GFP+LUC cells using CRISPR/Cas9-mediated

(11) Interactions between cancer cells and immune cells drive transitions to mesenchymal-like states in glioblastomaCancer cellJune 14, 2021Hara T, Chanoch-Myers R, Mathewson ND, Myskiw C, Atta L, Bussema L, Eichhorn SW, Greenwald AC, Kinker GS, Rodman C, et al.

(12) Blocking TNFα attenuates progressive cartilage matrix degradation in inflammatory arthritisExperimental and Therapeutic MedicineMay 27, 2021Jinsung Park, Hyosun Park, Young Lim Lee, Subin Weon, Yong-Gil Kim, Jae-Hyuk Yang, Bora Nam, Sungsin Jo, Tae-Hwan KimBioLegend, Inc.; 1:100), IgG1-FITC (cat. no. 400109; BioLegend, Inc.; 1:100) or IgG2a-APC (cat. no. 400221; BioLegend, Inc.; 1:100) for 30 min at 4˚C. The dilution of the antibody for FACS was ranged from 1:100-1:200.

(13) Thiopurines activate an antiviral unfolded protein response that blocks influenza A virus glycoprotein accumulationJournal of virologyMay 10, 2021Slaine PD, Kleer M, Duguay BA, Pringle ES, Kadijk E, Ying S, Balgi A, Roberge M, McCormick C, Khaperskyy DA.catalog number 311410; BioLegend) or an APC-conjugated mouse IgG2aκ isotype control (catalog number 400222; BioLegend). After staining, the cells were washed twice with PBS, fixed with 4% paraformaldehyde in

(14) Tofacitinib overcomes an IFNγ-induced decrease in NK cell-mediated cytotoxicity via the regulation of immune-related molecules in LC-2/adThoracic cancerMarch 1, 2021Okita R, Shimizu K, Nojima Y, Saisho S, Nakata M.

(15) Development of an Inflammatory CD14⁺ Dendritic Cell Subset in Humanized MiceFrontiers in immunologyJanuary 1, 2021Iwabuchi R, Ide K, Terahara K, Wagatsuma R, Iwaki R, Matsunaga H, Tsunetsugu-Yokota Y, Takeyama H, Takahashi Y.RRID:AB_2847829 Cat# 400125, RRID:AB_2861433 Mouse IgG2a kappa MOPC-173 APC PE BioLegend Cat# 400222 Cat# 400212, RRID:AB_326460 Rat IgG1 kappa RTK2071 PE BioLegend Cat# 400407, RRID:AB_326513 Isotype

(16) Endoderm and Hepatic Progenitor Cells Engraft in the Quiescent Liver Concurrent with Intrinsically Activated Epithelial-to-Mesenchymal TransitionCell transplantationJanuary 1, 2021Fagg WS, Liu N, Patrikeev I, Saldarriaga OA, Motamedi M, Popov VL, Stevenson HL, Fair JH.APC mouse anti-human CD99 (clone HCD99, BioLegend), or APC Mouse IgG2a, k isotype control (catalog # 400222, BioLegend). After staining and washing, the cells were passed through a 40 µm strainer and kept on

(17) Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cellseLifeDecember 17, 2020Gumber D, Do M, Suresh Kumar N, Sonavane PR, Wu CCN, Cruz LS, Grainger S, Carson D, Gaasterland T, Willert K.304008 1:100 RRID:AB_314396 Antibody APC mouse IgG2a k isotype control, mouse monoclonal Biolegend Cat# 400222 1:100 Antibody PE mouse IgG1, k isotype control, mouse monoclonal Biolegend Cat# 400112 1:100 RRID:AB_2847828 Antibody Alexa-Fluor

(18) Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridementeLifeDecember 2, 2020Kalinski AL, Yoon C, Huffman LD, Duncker PC, Kohen R, Passino R, Hafner H, Johnson C, Kawaguchi R, Carbajal KS, et al.Control-e450 (Biolegend, 400235), CD45.2-APC (Biolegend, 109813), Mouse IgG2ak Isotype Control-APC (Biolegend, 400221), Ly6G-APC-Cy7 (BD Biosciences, 560600), Rat IgG2a Isotype Control-APC-Cy7 (BD Biosciences, 552770),

(19) Immunoglobulin recognition of fecal bacteria in stunted and non-stunted children: findings from the Afribiota studyMicrobiomeJuly 27, 2020Huus KE, Rodriguez-Pozo A, Kapel N, Nestoret A, Habib A, Dede M, Manges A, Collard JM, Sansonetti PJ, Vonaesch P, et al.anti-human IgG Fc APC (BioLegend 409306) and isotype control (BioLegend Mouse IgG2a kappa isotype, 400222) were used at the same 1:25 dilution. Samples were washed twice more and fixed overnight in 2% paraformaldehyde

(20) TraPS-VarI: Identifying genetic variants altering phosphotyrosine based signalling motifsScientific reportsMay 21, 2020Ulaganathan VK.(BioLegend, Cat# 116417, Clone-AF6-120.1), APC Mouse IgG2a, κ Isotype Ctrl Antibody (BioLegend, Cat# 400221, Clone-MOPC-173), APC anti-mouse CD45 Antibody (eBioscience, Cat# 17-0451-82, Clone-30-F11), APC Armenian

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(1)

10/16/2015Good Isotype Control for APC Flow Antibody PENG SHIThis isotype control was used for the isotype control of APC conjugated flow antibody. It worked well with double staining with PE color too.

APC Mouse IgG2a, kappa isotype Ctrl (FC) from BioLegend

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APC Mouse IgG2a, kappa isotype Ctrl (FC) from BioLegend

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