Fig 2: LncRNA Gm13568 interacts with CBP/P300 to regulate the expression of Notch1 in astrocytes. a RIP assay was performed for the interaction of Gm13568 with NF-?B p65 and CBP/P300 in astrocytes treatment with IL-9 for 6 h. The data were from three independent experiments. b ChIP assay analysis of NF-?B p65, CBP/P300, RNA Pol II, and H3K27ac enrichment on the promoter of Notch1 gene in astrocytes stimulation with IL-9 for 6 h. *p < 0.05, **p < 0.01, ***p < 0.001 vs DMEM (one-way ANOVA). The data are from three independent experiments. Error bars represent mean ± SEM. c ChIP assay analysis of NF-?B p65, CBP/P300, and H3K27ac enrichment on the promoter of Notch1 gene in astrocytes infected by LV-Inhibit-Gm13568 for 72 h, followed by stimulation with IL-9 for 6 h. #p < 0.05 versus LV-ctrl. The data were from three independent experiments

Fig 3: Superposition of docked ligands in the active site of p300. The protein structure is shown in green cartoon representation, and the docked ligands—acetyl-CoA (green), carnosol (pink), C646 (yellow) and CPTH2 (orange) —are depicted in stick representation.

Fig 4: Model explaining how p-STAT1 interacts with EP300 to promote H3K27ac enrichment on the enhancers of LCP2 and TNFAIP2 and contributes to the development of chronic inflammation. When STAT1 is activated by IFN-?, STAT1 migrates into the nucleus and binds to the enhancers of LCP2 and TNFAIP2. p-STAT1 interacts with EP300 to promote H3K27ac enrichment on the enhancers and promote the transcriptions of LCP2 and TNFAIP2 and then contributes to the development of chronic inflammation

Fig 5: PBA enhances the acetylation of CBP/p300 and histone H3 at the CPT1A and INSIG2 promoters in Huh7-shTSC2 cells. PBA (8 mM, 6 h) induced the gene expression of CPT1A and INSIG2 (A), and the acetylation status of histone H3 and histone H4 (B). Chromatin immunoprecipitation (ChIP) assay revealed PBA-mediated enrichment of acetyl CBP/p300 (Ac-p300) and acetyl histone H3 (Ac-H3) at the CPT1A and INSIG2 promoters (C). Statistical significance was determined using Student’s t-test, * p < 0.05, n = 4.