Fig 1: Inflammatory signal upregulates Gp2 expression in pancreatic acinar cells.a Cultured pancreatic acinar cells are shown. b Pancreas of control (upper) or Ptf1aCre-ERTMCre-tdTomato (bottom) mice received tamoxifen are shown. c Cultured pancreatic acini from control of Ptf1aCre-ERTM-tdTomato mice stimulated with 4OHT are shown. Data are representing for three independent experiments. d Gp2 expression in no stimulation (None) or stimulated by 200 ng/mL LPS, 20 ng/mL TNF, 20 ng/mL IL-6 (n = 10 /group), 100 ng/mL RANKL (n = 4) pancreatic acini were examined. Relative expressions mRNA levels were determined by quantitative RT-PCR and normalized to Gapdh. *p = 0.040 (one-way ANOVA). Data are presented as mean values ± SEM., N.S. indicates not significant. e Pancreas of mice systemically administered PBS (control) or TNF (100 ng, two times). GP2 (red), DAPI (blue). Representative data of three independent experiments are shown. f GP2 concentration in pancreatic juice. *p = 0.04 (two-tailed Mann–Whitney U test). Data are presented as mean values ± SEM. g TNF concentration in serum *p = 0.021 (two-tailed unpaired t-test). Data are presented as mean values ± SEM. h Representative photograph showing the abdominal-organ matrices surrounding the pancreas in Ptf1aCre-ERTM-tdTomato mice that received tamoxifen. i TNF concentration in abdominal-organ matrices, as determined by enzyme-linked immunosorbent assay. Pancreas (intact n = 15, colitis n = 13), Omentum (intact n = 10, colitis n = 11) mesenteric fat (intact n = 15, colitis n = 13), Retroperitoneal fat and Epididymis fat (intact n = 3, colitis n = 3) *p < 0.05 (two-tailed unpaired t-test). Data are presented as mean values ± SEM. j 100 µg of anti-TNF antibody (Biolegend, MP6-XT22, #506332) or Isotype Rat IgG2a (Biolegend, RTK2758, #400503) was intraperitoneal administered three times (on days 1, 3, and 5) during DSS treatment, and pancreases were collected on day 6. GP2 (red), DAPI (blue). Representative data of three independent experiments are shown. Source data are provided as a Source Data file.