Fig 2: Sec61 blockade by mycolactone strongly alters the biology and viability of MM cell lines AEffect of mycolactone on MM.1S, JIM3, and KMS-11 cell viability after 48 and 72 h of treatment, as measured by exposure of Annexin V and incorporation of PI. Data are Mean % of live, apoptotic, and dead cells from technical duplicates, gated as in Appendix Fig S1, relative to total cells.B, CInhibitory effect of mycolactone on cell expression of CD38 (Type II) and CD138, IL-6R, and CD40 (Type I) transmembrane proteins (B). Stimulatory effect of mycolactone on cell expression of BCMA, a Type III transmembrane protein (C). Mean fluorescence intensities (MFIs) were measured in live cells 48 h after addition of mycolactone. Data are Mean MFIs ± SD from technical duplicates of two independent experiments, relative to vehicle-treated controls (Ctrl).DInhibitory effect of mycolactone on immunoglobulin (lambda chain) secretion, as quantified by ELISA in culture supernatants after 24 h of treatment. Data are Mean concentrations ± SD of secreted lambda chain (µg/ml) from technical duplicates of two independent experiments (MM.1S) or Mean of secreted lambda chain concentrations (µg/ml) from technical duplicates (JIM3 and KMS-11), relative to vehicle-treated controls.ECorrelation between immunoglobulin lambda chain (Ig) secretion after 24 h (D) and MM cell line mortality after 72 h (A), in the three cell lines treated with 50 nM mycolactone. Slopes (R) and statistical significance (P values) are indicated. Data information: In A (all cell lines) and D (JIM3 and KMS-11), shown data are representative of two independent experiments with similar results.