Fig 2: Increase in SNAP25-containing neurites in PC12 cells by ACD856. PC12 cells were treated with 3 ng NGF/mL for 5 days in the absence or presence of increasing concentrations of ACD856. Cells were fixed with PFA, and anti-SNAP25 (red) or anti-beta tubulin (green) antibodies were used to visualize cells and neurites. A dose–response effect of ACD856 was observed for SNAP25-positive neurite total count per neuron (a). The SNAP25-protein was mainly localized to the cell body, neurites, and especially in buddings of neurites or at nerve endings (indicated with arrows). Image was acquired with ×20 magnification. (b). ** p-value < 0.01, compared to control group at one-way ANOVA with Dunnett’s multiple comparisons test. Data shown are the mean of average values from individual experiments +/- SD (n = 3).