Fig 1: Gas6 does not affect microglial phagocytosis of amyloid beta in vivo or in vitro. A Ten-month-old APP/PS1 and nontransgenic littermates injected with AAV-Gas6 or AAV-control at 9 months of age were intraperitoneally injected with MX-04 24 h prior to sacrifice. Brains were processed and microglia (CD45lo/CD11b+) were analyzed by flow cytometry. Representative plots of gating strategy. B, C MX-04 + microglial populations showing percent of MX-04+ cells and relative median fluorescence per cell. N = 2–4 per group. Error bars represent mean ± SEM. D Primary microglia from Axl+/- mice were treated with rmGas6 (50 nM) and beta-amyloid (1–42) HiLyte Fluor 488 for 15 or 30 min and analyzed by flow cytometry. N = 4–5 biological replicates per group. Error bars represent mean ± SEM. E Primary microglia from Axl + / - or Axl-/- P0-P2 pups were treated with rmGas6 for 15, 30, 60, or 120 min and analyzed by flow cytometry. N = 4–6 biological replicates per group. Error bars represent mean ± SEM