Immunofluorescence: eGFP Antibody [NBP2-59183] - A mitotic human HeLa cell, stably expressing a GFP-CENP-A fusion protein, was fixed with 4% formaldehyde and processed for immunofluorescence using the antibody against EGFP, diluted 1:800, and a monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody, respectively. DNA was visualized using Hoechst staining. Images of the cell were taken with a Deltavision microscope. The images shown in Figure represent a projection of approximately 30 0.2 um optical sections.
eGFP Antibody
Supplier Page from Novus Biologicals, a Bio-Techne Brand for eGFP Antibody