Fig 1: Effects of low-dose lithium supplementation on soleus and extensor digitorum longus (EDL) NFAT activation, PGC-1a and MHC isoform expression. (a) Representative Western blot images of phosphorylated and total nuclear factor activated T cells (p-NFATc1 and NFATc1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1-a), all of which are downstream markers of calcineurin signaling. For pNFATc1, NFATc1, and PGC-1a, 7.5 µg of total protein was loaded into each well. (b) Quantification of p-NFATc1 and NFATC1 were first normalized to ponceau prior to calculating the p-NFATc1/NFATc1 ratio. (c) Quantification of PGC1-a content in soleus and EDL muscles in control-fed and LiCl groups normalized to ponceau. (d) Representative Western blot images of MHC I and IIa in soleus muscles from control and lithium-fed mice. For both isoforms, 5 µg of total protein was loaded into each well. Quantification of MHC I (e) and MHC IIa (f) normalized to ponceau. For (b,c) and (e,f), all data are expressed relative to control. *p < .05, significantly different from control, using a Student's t tests (n = 6–9 per group). All data are expressed as means ± SD