Fig 1: Insulin resistance does not impair overload-stimulated increases in glucose transporter protein levels. After 12 weeks of high-fat diet (HFD) feeding, mice underwent unilateral synergist ablation surgery to induce overload (OVL) of the plantaris muscle. Five days later, muscles were processed for immunoblot (IB) analysis of glucose transporter 1 (A: GLUT1); (B) GLUT3; (C) GLUT4; (D) GLUT6; (E) GLUT10; and (F) hexokinase II (HXII). Data are mean ± standard deviation. Statistical analysis was performed using a 2-way ANOVA with Tukey’s post-hoc analysis. N = 7–10 muscles/group. p < 0.05 # vs. Sham (Sh); * vs. low-fat diet (LFD); @ = main effect.