Fig 1: (A) Top six minoxidil-related derivatives exhibited neuroprotective effects on synaptogenesis or neurite outgrowth in primary cortical neurons against paclitaxel-induced neurotoxicity. Data represent the mean ± S.E.M. of at least three independent experiments. Statistical significance was analyzed using two-tailed Student’s t-test. *P < 0.05; **P < 0.01 vs paclitaxel treatment. (B) Representative images (top panels) show primary cortical neurons costained for the microtubule-associated protein 2 (MAP2, green) and synaptophysin (red) following various treatment regimens. The binary mask image (middle and bottom panels) was created from the MAP2 and the synaptophysin fluorescent signal. Scale bar, 100 µm.