Fig 1: Age-dependent output of blood td-ILCs and their differentiation(A) Peripheral blood of indicated strains of mice were analyzed. The frequencies of Lin−Thy1+CD127+ and subsequent CD62L+icCD3ε+ subsets are quantified.(B) The average frequency of blood CD62L+icCD3ε+ cells of the Lin−Thy1+CD127+ compartment in WT mice of indicated ages. Data are represented as mean ± SD. One-way ANOVA was used for determining the statistical significance of data shown in (A) and (B). ∗p<0.05, ∗∗<0.01, ∗∗∗p<0.001, ∗∗∗∗p<0.0001, ns, not significant.(C) In vitro differentiation on OP9-DL stroma of the indicated cell populations (800 cells per well) in 30 ng/mL of each IL-2, IL-7, and stem cell factor. Cells were analyzed on day 9 of the culture and the frequencies of indicated subsets are shown in the graphs. Data shown are representative of three independent experiments.