Fig 2: SDS-PAGE and immunoblot analysis using an antibody against CKB in mouse lenses. (A) Immunoblot analysis of CKB in wild-type (WT), cryaa-R49C-heterozygous, and cryaa-R49C-homozygous mutant lenses. CKB immunoreactivity is indicated by the top arrow. The lower molecular weight bands were apparently due to the cross reactivity of the secondary antibody with crystallins (lower arrows). (B) Quantification of the CKB band observed at approximately 40 kDa in (A). Equal amount of protein was loaded in each lane. Note the increase in expression of CKB in the cryaa-R49C homozygous lenses.

Fig 3: ELISA assay for CKB activity in mouse lenses. (A) CKB activity in wild-type (WT) and R49C mutant mouse lenses. 1–2-month-old mice were used. The enzyme activity was normalized to a value of 100% for WT lenses. Data from three or more lenses of each genotype is shown. WT vs. cryaa-R49C-/-, p < 0.05.