Fig 1: Reduced cytoophidium formation is dependent on mTORC1. A: SW480 cells expressing shRNA targeting mTOR, Raptor, Rictor, or RFP (as control) were analyzed by immunofluorescence staining for the presence of CTPS cytoophidium. B: Quantitative data of percentage of cells with cytoophidia shown in (A). C: Western blotting analysis of mTOR, Raptor and Rictor protein expression in the cells expressing shRNA of the indicated genes. D: SW480 cells transfected with siRNA targeting mTOR, Raptor, Rictor, or scrambled control siRNA were subjected to immunofluorescence staining with anti-CTPS antibody. E: Quantitative data for SW480 cells with CTPS cytoophidia shown in (D). F: Western blotting analysis of mTOR, Raptor and Rictor protein expression in cells transfected with scrambled control siRNA or siRNA targeting mTOR, Raptor or Rictor with appropriate antibodies. ß-Actin was used as a loading control in (C) and (F). Mean ± S.E.M., n.s., not significant; *P < 0.05, **P < 0.01; ****P < 0.0001 versus control. Scale bars = 20 µm. One of four to six similar experiments is shown.