Fig 1: Classification and analysis of epithelial cells in cystitis glandularis. (A) U-MAP plot of different subsets of epithelial cells correlated by color. (B) Heat map showing Top5-specific genes expressed in epithelial cell subsets. (C) InferCNV was used to estimate the copy number variation score of epithelial cells, in which CG and normal bladder mucosa were compared to endothelial cells and fibroblasts. (D) The proportion of different kinds of epithelial subsets among all epithelial cells. The left panel indicates normal tissue and the right panel indicates inflamed tissue. (E) Pseudotime trajectory of epithelial cells of all samples inferred by Monocle2. Bottom: (left) pseudotime trajectory of epithelial cell subsets marked by color, and (right) the chronological order of development marked by color depth. Top: Change curve of the overall expression content of the superficial epithelial cell marker gene set based on the pseudotime trajectory of epithelial cell subsets. (F) Heat map showing the expression of major transcription factors in epithelial cell subsets using SCENIC. (G) Multiplex immunofluorescence verified various kinds of epithelial cell infiltration. (H) TNF-P65 inflammatory activation in KRT15 epithelial cells.