Fig 1: PIP promotes autophagic flux by enhancing autophagosome-lysosome fusion via P2RX4 activation. (A) STX17 level was elevated as shown by western blotting in SK-N-SH cells. (B) Quantitative analysis of STX17 level in SK-N-SH cells. The normalized ratio for the con group was taken as 1. Data are expressed as means ± SD (one-way analysis of variance). #P < 0.05 vs. con; *P < 0.05 vs. SNCA; &P < 0.05 vs. SNCA+PIP (n = 3). (C) LC3-II, SQSTM1/p62 and STX17 levels were elevated as shown by western blotting of OB. (D–F) Quantitative analysis of LC3-II (D), SQSTM1/p62 (E) and STX17 (F) levels in OB. ACTB was used as a loading control. The normalized ratio for the WT group was taken as 1. Data are expressed as means ± SD (one-way analysis of variance). #P < 0.05 vs. WT; *P < 0.05 vs. TG (n = 3).