Fig 1: Effects of DS1 on IL-18 and IL-1ß. (A) Compared to NS control, increased levels of IL-18 binding protein (BP) bound and total IL-18 (pro + mature + bound to IL-18BP) were seen in the DS1 group by Western blot. Identity of the IL-18BP band was confirmed by reacting samples with IL-18BP antibody (middle blot). Bar graph to the right below shows band densities for all forms of IL-18 (all bands) normalized to beta-actin (n = 3 cornea per group, fold change *P = 0.05). (B) IL-18 immunostaining in whole-mount corneas from NS and DS1, n = 3 per group. Nuclei are stained blue with Hoechst. (C) Western blot of pro and mature forms of IL-1ß. Bar graph below the blot shows pro and mature band densities at each timepoint. Decreased pro-IL-1ß compared to NS and increased mature IL-1ß compared to DS1 was seen in the DS5 group (*P = 0.05). Bar graph to the right below shows pro/mature IL-1ß ratios (n = 4 corneas per group, fold change *P = 0.05). (D) Expression of il18, il18bp and il1ß measured by real time PCR at NS and DS1, (fold change *P = 0.05).