Fig 1: miR-33-5p suppresses the JNK pathway by directly inhibiting the expression of JNK1. DF-1 cells were transfected with the indicated RNA oligonucleotides and infected with MG. JNK1, JUN and FOS mRNA levels and protein levels were measured by qPCR and Western blotting, respectively. (A) The relative mRNA expression level of JNU and FOS after treatment with miR-33-5p mimics; (B) The relative mRNA expression level of JNU and FOS after treatment with miR-33-5p inhibitor; (C) The protein expression of JNU and FOS after treatment with miR-33-5p mimics; (D) The protein expression of JNU and FOS after treatment with miR-33-5p inhibitor; (E) The relative mRNA expression level of JNK1, JUN and FOS after co-transfection of overexpression JNK1 vector and miR-33-5p mimics; (F) The relative mRNA expression level of JNK1, JUN and FOS after co-transformation of SP600125 and miR-33-5p inhibitor; (G) The protein expression of JNK1, JUN and FOS after co-transfection of overexpression JNK1 vector and miR-33-5p mimics; (H) The protein expression of JNK1, JUN and FOS after co-transformation of SP600125 and miR-33-5p inhibitor. GAPDH was used as the internal control. (*p < 0.05, **p < 0.01).