Fig 1: Disruption of ITPR2 increases the proportion of type I/II oligodendrocytes. (A–C) Double immunostaining of CAII and CC1 in early postnatal brain tissues. (D) Quantification of the density of CC1 positive cells and CAII/CC1 double positive cells in the corpus callosum of wild-type mice. (E–G') Immunofluorescent images of CAII and SOX10 staining in corpus callosum tissues from various stages of WT and Itpr2-/mice. Double positive cells are represented by white arrows. (H) Quantitative analysis of the ratio of CAII+ cells in SOX10+ oligodendrocytes at indicated stages. (I–K') CAII and SOX10 double-immunostaining in corpus callosum tissues from various stages of control and Cnpcre/+; Itpr2fl/fl mice. Double positive cells are represented by white arrows. Scale bar represents 50 µm. (L) Quantitative analysis of the percentage of CAII+ cells in SOX10+ oligodendrocytes at indicated stages. (M) Schematic of workflow of organotypic slice cultures. (N,N') Immunostaining of CAII in the corpus callosum of cultured Sox10-GFP slices. Compared with the control groups, the ratio of CAII+SOX10+ oligodendrocytes remarkably increased in the corpus callosum of cultured slices treated with 20 µM BAPTA-AM. Scale bar represents 50 µm. (O) Quantification of the percentage of CAII/SOX10-GFP double positive cells in SOX10-GFP+ population in brain slice culture from (N,N'). Error bar indicates Means ± SEM. n = 3.