Fig 1: Expression levels of ETC complex subunits and PGC1-NRF1/2 signaling pathway proteins during differentiation of C2C12 myoblasts. (a) Western blot of ETC complex subunits ND1, CYTB, ATP6A1, SDHA, and COXIV in C2C12 cells during differentiation on D1, D3, and D5 after treatment with UCF101 (20 µM) or no treatment and quantification of Western blots relative to GM (n = 3). (b) Western blot of PGC1a, NRF1, NRF2, and VDAC in C2C12 myoblasts at differentiation on D1, D3, and D5 after treatment with UCF101 (20 µM) or no treatment and quantification of Western blots relative to GM (n = 3). Two-tailed unpaired Student’s t-tests were used. Statistical significance: * p = 0.05, ** p = 0.01. ND1, NADH1; SDHA, succinate dehydrogenase complex flavoprotein subunit A; COXIV, cytochrome c oxidase subunit 4; CYTB, cytochrome b; ATP6, ATP synthase F0 subunit 6.