Fig 1: The posttranslational modification of Nrf2 stimulated by virus infection. MDBK cells in 60 mm dishes were mock infected or infected with BoHV-1 (MOI = 0.1) for 24 h; the cell cultures were collected to produce whole cell lysates (a and d) or to extract nuclear proteins using commercial nuclear protein purification kit (Beyotime Biotechnology, cat# P0027) (b and c). IP was performed using the antibody against Nrf2 (Abcam, cat# ab137550). Then, levels of phospho-Nrf2 in whole cell extracts (WCE) (a) and the nucleus (b) and acetylation levels of Nrf2 in the nucleus (c) and WCE (d) were detected by immunoblots using the antibody against the Nrf2 antibody (Abcam, cat# ab1375501:500), acetyl Lysine antibody (ABclonal, cat# A2391, 1 : 1000), and phospho-(Ser/Thr) Phe antibody (Cell Signaling Technology, cat# 9631, 1 : 1000). Data shown are representative of three independent experiments. The significance was assessed with Student's t-test (*P < 0.05).