Fig 1: DdCBE-mediated KO of rat mtProteins in C6 cells.(A) Strategy of mtDNA-encoded protein depletion by DdCBE-introduced stop codon. The W codon TGA was changed to TAA stop codon (W stop). The Q codon CAA was changed to TAA stop codon (Q stop). (B) Location of introduced stop codon at mtProteins. (C to L) The identification of DdCBE-mediated mtProtein depletion in ND2-KO (C), ND3-KO (D), ND4-KO (E), ND5-KO (F), ND6-KO (G), CYTB-KO (H), COX1-KO (I), COX2-KO (J), COX3-KO (K), and ATP6-KO (L) C6 cells by deep sequencing (left), Western blot (middle), and Seahorse assay (right). For deep sequencing, n = 3 technical replicates. For Seahorse assay, n = 5 technical replicates for Ctrl and n = 6 technical replicates for each KO cell line. Data were presented as means ± SD.