CFTR Antibody (CFTR/1643) [Alexa Fluor® 647] NBP2-54509AF647 from Novus Biologicals, a Bio-Techne Brand

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European Archives of Oto-Rhino-Laryngology
July 01 2023

Fig 1: a To remove the inflammatory cells from the exfoliated cells in the nasal cavity, CD45-negative cells were screened for analysis. Blue represents non-inflammatory cells and red represents inflammatory cells. FOXI1 positive cells are ionocytes, Q1 is non-ionocytes that express CFTR, Q2 is ionocytes that express CFTR, and Q4 is ionocytes that do not express CFTR. Comparing the number of cells expressing CFTR and the number of ionocytes between the two groups, there was no significant difference in CFTR, but there was a significant decrease in the number of ionocytes and CFTR–FOXI1 co-expression cells in the CRS group. b P2 is non-inflammatory cells and P3 is CFTR-positive cells. The combination of MQAE dye and Cl- will emit green fluorescence, and the fluorescence intensity represents the concentration of Cl- in cells. CFTR mediates the extracellular excretion of Cl-, so the higher the intracellular fluorescence intensity is, the lower the CFTR function is. Comparing the intracellular fluorescence intensity among the three groups, it was found that the intracellular fluorescence intensity was the highest in the CRS group and the lowest in the nasal bone fracture over 14 day group, indicating that the CRS patients had acquired CFTR dysfunction
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European Archives of Oto-Rhino-Laryngology
July 01 2023

Fig 2: a Distribution of ionocytes in the nasal mucosa and the polyp epithelium was uneven and clustered between pseudostratified ciliated columnar epithelial cells. Scale bar = 25 µm; The cell is "tadpole-shaped", the tail end narrows and extends to the free edge of the ciliated cells on the epithelium surface. Scale bar = 100 µm. b Self-paired comparison of the number of cells co-expressed by CFTR and FOXI1 in CRSwNP patients. The histogram represents the number of ionocytes in the nasal epithelium, and the dot represents the number of CFTR–FOXI1 co-expression cells. c Ionocytes that do not express CFTR are more common in the polyp epithelium, and these ionocytes do not show a "tadpole" shape. Scale bar = 100 µm
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European Archives of Oto-Rhino-Laryngology
July 01 2023

Fig 3: a Number of CFTR-expressing cells in the nasal mucosa and nasal polyp epithelium. The number of CFTR fluorescence was used to represent the expression of CFTR and scored. The number of CFTR-0 and CFTR-1 cells in the nasal mucosa and polyp epithelium was the highest, while the number of CFTR-2 and CFTR-3 cells was lower, and it was more obvious in the polyp epithelium. b Distribution of CFTR and ionocytes in the nasal mucosa and polyp epithelium. Scale bars = 50 µm. c Statistical analysis of the expression of CFTR and the number of ionocytes in the nasal epithelium. Ionocytes were denser in the mucosa of diffused CRS and CFTR was denser in polyps of diffused CRS