Fig 2: Flow Panel characterizing CD11b, CD45, CX3CR1, CD68, CD317 and CD169 for the different microglial cell types and MDMs as labelled on the left. Blue histogram represents isotype-control antibodies, red histograms represent the specific staining for markers indicated at the top of the figure

Fig 3: Human microglia model expression profile of selected factors related to HIV-1 biology. a Histograms displaying cell surface expression of CD4, CXCR4, CCR5, CD317, and CD169 for the four microglial model cells and MDM. Isotype controls are shown in blue, specific staining at baseline in red. IFN stimulation was pursued for CD317 and CD169, with post-stimulation histograms depicted in green. b Heatmap of HIV-1 receptor, coreceptor and restriction factor gene expression, with color scale of expression levels indicated

Fig 4: Proliferative potential of hiPSC/SEAM-derived BST2+ cells(A) Schematic of the differentiation and sorting method for the cells.(B) Flow cytometric analysis of CD200, CD104, and BST2 for the EGF-treated SEAM derivative at week 12. CD200- cells were extracted first, and then the P1–P4 fractions were sorted based on CD104 and BST2 expression. P1–P4 fractions are defined as follows: (P1) CD200-, CD104+, and BST2+ cells; (P2) CD200-, CD104+, and BST2-cells; (P3) CD200-, CD104-, and BST2+ cells; (P4) CD200-, CD104-, and BST2- cells.(C) Representative images of colony-forming assays of sorted cells from P1, P2, P3, and P4. n = seven independent experiments.(D) Colony-forming efficiency of sorted cells from P1 and P2. n = 7. *p < 0.05. Error bars show the standard deviation.(E) Gene expression analysis of ocular surface epithelial cell markers in the EGF-treated SEAM derivative at week 12 and in P1, P2, P3, and P4 cells. n = 7. *p < 0.05. Error bars show the standard deviation. See also Figures S3 and S4.

Fig 5: Fabrication of epithelial sheets from hiPSC/SEAM-derived BST2+ cells(A) Schematic of the method of generating epithelial sheets from the cells.(B) Phase-contrast images of the sheet fabricated from sorted BST2+ (P1) and BST2- (P2) cells. n = three independent experiments. Scale bars; 100 µm.(C) Immunostaining of MUC5AC, K13, and K12 (green) in the sheet derived from P1–P2 cells. n = three independent experiments. Nuclei, red. Scale bars; 25 µm.(D) Gene expression analysis of ocular surface epithelial cell markers for sorted cells from P1–P2 (FACS). n = 7. *p < 0.05. Error bars show the standard deviation.(E) Gene expression analysis of ocular surface epithelial cell markers for the sheet derived from P1–P2 (Sheet). n = 7. *p < 0.05. Error bars show the standard deviation. See also Figure S4.