Ubiquityl-Histone H2A (Lys119) (D27C4) XP ® Rabbit mAb 8240T from Cell Signaling Technology

Product Specs
ItemUbiquityl-Histone H2A (Lys119) (D27C4) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number8240T
Quantity20 µl
ApplicationsW, IP, IF-IC, FC-FP, ChIP, ChIP-seq, C&R, C&T
ReactivityH, M, R, Mk
TargetH2A.4 (Lys119) ubiquitinate
IsotypeIgG
HostRabbit
NCBI Full Gene NameH2A clustered histone 1
NCBI Gene AliasesH2AA, H2AFR, HIST1H2AA, HISTH2AA, TH2A, bA317E16.2
ClonalityMonoclonal
Research AreaChromatin Regulation / Nuclear Function
Molecular Weight23

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Cell Signaling Technology

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Phone: 978-867-2388
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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Rbp4-Gal4, a germline driver that activates in meiosis, reveals functions for VCP in spermatid developmentFly (Austin)December 31, 2023Tyler J. Butsch, Alyssa E. Johnson, K. Adam Bohnertwere used in this study: rabbit anti-GFP (1:1000; Invitrogen A21311), rabbit anti-H2Aub (1:100; CST #8240S) and goat anti-rabbit 488 (1:500; Invitrogen A11034). Images were acquired using an inverted Leica SP8

(2) Chromosome-specific maturation of the epigenome in the Drosophila male germlineeLifeNovember 30, 2023James T Anderson, Steven Henikoff, Kami Ahmadanti-H3-K4-dimethyl, Cell Signalling Technology E1Z3G anti-RNAPII-Serine-2-phosphorylation, Cell Signalling Technology 8240 anti-H2A-K119-ubiquitinylation, EMD Millipore 05-1249 anti-H3-K9-dimethyl, Cell Signalling Technology

(3) KDM2B regulates hippocampal morphogenesis by transcriptionally silencing Wnt signaling in neural progenitorsNature CommunicationsOctober 14, 2023Bo Zhang, Chen Zhao, Wenchen Shen, Wei Li, Yue Zheng, Xiangfei Kong, Junbao Wang, Xudong Wu, Tao Zeng, Ying Liu, et al.immunoprecipitation was further performed with sheared chromatin and 3 μg rabbit anti-H2AK119Ub antibody (CST, 8240 S); or rabbit anti-H3K27me3 antibody (CST, 9733S); or rabbit anti-H3K36me2 antibody (CST, 2901S), then

(4) CBX4 promotes antitumor immunity by suppressing Pdcd1 expression in T cellsMolecular OncologyOctober 9, 2023Liwei Ren, Ziyin Li, Yu Zhou, Jun Zhang, Ziheng Zhao, Zhaofei Wu, Ye Zhao, Yurong Ju, Xuewen Pang, Xiuyuan Sun, et al.ratio 1 : 1000), anti‐H3K27me3 (9733S; clone: C36B11; antibody ratio 1 : 5000), and anti‐H2AK119ub1 (8240S; clone: D27C4; antibody ratio 1 : 5000) were purchased from Cell Signaling Technology. 2.12 Statistical

(5) Derepressing of STAT3 and USP7 contributes to resistance of DLBCL to EZH2 inhibitionHeliyonOctober 5, 2023Chenyun He, Wenbin Zhou, Xiaoxia Jin, Haining Zhou1:200). H3K27me3 (Cell Signaling Technology,9733S, WB 1:200). H2AK119ub1 (Cell Signaling Technology, 8240S, WB 1:1000). H3 (Abcam, ab1791, WB 1:1000). H2A (Active Motif, 39945, WB 1:100). GAPDH (Cell Signaling Technology

(6) PR‐DUB safeguards Polycomb repression through H2AK119ub1 restrictionCell ProliferationOctober 1, 2023Rui Li, Dandan Huang, Yingying Zhao, Ye Yuan, Xiaoyu Sun, Zhongye Dai, Dawei Huo, Xiaozhi Liu, Kristian Helin, Mulin Jun Li, et al.dialyzed to lower SDS concentration to 0.01% followed by standard IP assays with H2AK119ub1 antibody (CST 8240S). After reversal of the crosslinking, ChIP DNA was purified for qPCR analysis. 2.6 H2AK119ub1 CUT&Tag WT

(7) INHAT subunit SET/TAF-Iβ regulates PRC1-independent H2AK119 mono-ubiquitination via E3 ligase MIB1 in colon cancerNAR CancerSeptember 22, 2023Junyoung Park, Ji-Young Kim, Jin Woo Park, Joo Young Kang, Hyein Oh, Ja Young Hahm, Yun-Cheol Chae, Debabrata Chakravarti, Sang Beom Seo1:1000) and β-actin (sc-47778, 1:1000) from Santa Cruz Biotechnology (Dallas, TX, USA); H2AK119ub (8240S, 1:5000), H3K4me3 (9751S, 1:5000) and H3K27me3 (9733S, 1:5000) from Cell Signaling Technology (Danvers,

(8) Aberrant gene activation in synovial sarcoma relies on SSX specificity and increased PRC1.1 stabilityNature structural & molecular biologySeptember 21, 2023Benabdallah NS, Dalal V, Scott RW, Marcous F, Sotiriou A, Kommoss FKF, Pejkovska A, Gaspar L, Wagner L, Sánchez-Rivera FJ, et al.min, 10 min and 20 min, respectively. The primary antibodies H2AK119ub1 (Cell Signaling Technology, 8240) and SSX-SS18 (Cell Signaling Technology, 72364S) were incubated at ambient temperature at 1:400 for

(9) Multi-omics comparison of malignant and normal uveal melanocytes reveals molecular features of uveal melanomaCell ReportsSeptember 13, 2023David Gentien, Elnaz Saberi-Ansari, Nicolas Servant, Ariane Jolly, Pierre de la Grange, Fariba Némati, Géraldine Liot, Simon Saule, Aurélie Teissandier, Deborah Bourc’his, et al.,Cell Signaling Technology,Cat# 9751; RRID: AB_2616028 "anti H2AK119ub, clone D27C4",Cell Signaling Technology,Cat# 8240; RRID: AB_10891618 anti H3K27Ac,Diagenode,Cat# C15410196; RRID: AB_2637079 anti CTCF,Diagenode,Cat#

(10) 1,25(OH)(2)D(3) ameliorates doxorubicin‑induced cardiomyopathy by inhibiting the NLRP3 inflammasome and oxidative stressExperimental and therapeutic medicineSeptember 1, 2023Gu X, Zhao L, Ye J, Chen L, Sui C, Li B, Wang X, Zhang J, Du Y.no. 9003; Cell Signaling Technology, Inc.) following the manufacturer's protocol. H2AK119Ub (cat. no. 8240), H3K4me3 (cat. no. 9751) and H3K27me3 (cat. no. 9733) were purchased from Cell Signaling Technology

(11) Structural basis of histone H2A lysine 119 deubiquitination by Polycomb repressive deubiquitinase BAP1/ASXL1Sci AdvAugust 9, 2023Jonathan F. Thomas, Marco Igor Valencia-Sánchez, Simone Tamburri, Susan L. Gloor, Samantha Rustichelli, Victoria Godínez-López, Pablo De Ioannes, Rachel Lee, Stephen Abini-Agbomson, Kristjan Gretarsson, et al.Western blots were performed with anti-BAP1 [D7W70, Cell Signaling Technology (CST)], anti-H2AK119ub1 (8240, CST), anti-H3 (ab1791, Abcam). Quantification and statistical analysis Protein quantification was

(12) Deficiency of the Polycomb Protein RYBP and TET Methylcytosine Oxidases Promotes Extensive CpG Island Hypermethylation and Malignant TransformationCancer researchAugust 1, 2023Cui W, Huang Z, Jin SG, Johnson J, Lau KH, Hostetter G, Pfeifer GP.8.0, 0.5 mmol/L EGTA, pH 8.0, for 8 minutes. For H2AK119ub1 ChIP (Cell Signaling Technology, antibody 8240) and H3K27me3 ChIP (Cell Signaling Technology, antibody 9733), cells were cross-linked with 1% formaldehyde

(13) Structural basis of histone H2A lysine 119 deubiquitination by Polycomb Repressive Deubiquitinase BAP1/ASXL1bioRxivJuly 31, 2023Jonathan F. Thomas, Marco Igor Valencia-Sánchez, Simone Tamburri, Susan L. Gloor, Samantha Rustichelli, Victoria Godínez-López, Pablo De Ioannes, Rachel Lee, Stephen Abini-Agbomson, Kristjan Gretarsson, et al.ChemiDoc Imaging System (Bio-Rad). Western blots were performed with: anti-BAP1 (D7W70; CST), anti-H2AK119ub1 (8240; CST), anti-H3 (ab1791; Abcam). Quantification and statistical analysis Protein quantification was

(14) Proximity-dependent recruitment of Polycomb repressive complexes by the lncRNA AirnCell reportsJuly 25, 2023Braceros AK, Schertzer MD, Omer A, Trotman JB, Davis ES, Dowen JM, Phanstiel DH, Aiden EL, Calabrese JM.RESOURCE,SOURCE,IDENTIFIER ,, Antibodies,, ,, H3K27me3,Cell Signaling,Cat#9733; RRID: AB_2616029 H2AK119ub,Cell Signaling,Cat#8240; RRID: AB_10891618 RING1B,Cell Signaling,Cat#5694; RRID: AB_10705604 CBX7,Abcam,Cat#ab21873; RRID:

(15) Early chromosome condensation by XIST builds A-repeat RNA density that facilitates gene silencingCell reportsJuly 25, 2023Valledor M, Byron M, Dumas B, Carone DM, Hall LL, Lawrence JB.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Anti-H3K27me3,Millipore Sigma,Cat# 07–449; RRID:AB_310624 Anti-UbH2A,Cell Signaling,Cat# 8240; RRID:AB_10891618 Anti-FK2 Ubiquitinylated Proteins,Affiniti,Cat# PW 8810 Anti-H4K20me,Abcam,Cat#

(16) Transcriptional regulation of amino acid metabolism by KDM2B, in the context of ncPRC1.1 and in concert with MYC and ATF4bioRxivJuly 15, 2023Evangelia Chavdoula, Vollter Anastas, Alessandro La Ferlita, Julian Aldana, Giuseppe Carota, Mariarita Spampinato, Burak Soysal, Ilaria Cosentini, Sameer Parashar, Anuvrat Sircar, et al.H3K27me3 1:50 (Cell Signaling Technology, Cat 9733), H2AK119ub 1:100 (Cell Signaling Technology, Cat 8240), H3K27ac (Cell Signaling Technology, Cat 8173). 2% input was used as control. ChIP-qPCR. The immunoprecipitated

(17) VCP promotes tTAF-target gene expression and spermatocyte differentiation by downregulating mono-ubiquitylated H2ADevelopmentJuly 15, 2023Tyler J. Butsch, Olga Dubuisson, Alyssa E. Johnson, K. Adam BohnertA21311), mouse anti-GFP (1:200; Invitrogen, A11120), rabbit anti-H2Aub (1:100; Cell Signaling Technology, 8240S), rabbit anti-VCP (1:100; Cell Signaling Technology, 2648), rabbit anti-mCherry (1:100; Invitrogen,

(18) Epigenetic plasticity safeguards heterochromatin configuration in mammalsNucleic acids researchJuly 7, 2023Fukuda K, Shimi T, Shimura C, Ono T, Suzuki T, Onoue K, Okayama S, Miura H, Hiratani I, Ikeda K, et al.immunoprecipitation reaction, 0.5 μl of rabbit monoclonal anti-H2AK119 monoubiquitylation antibody (Cell Signaling, #8240) conjugated to precleared Dynabeads Protein A and G mixture was used. After immunoprecipitation at 4°C

(19) Impaired histone inheritance promotes tumor progressionNature communicationsJune 10, 2023Tian C, Zhou J, Li X, Gao Y, Wen Q, Kang X, Wang N, Yao Y, Jiang J, Song G, et al.Technology, Cat.#5326), H3.3 (proteintech, Cat.#13754-1-ap), H2AK119Ub (Cell Signaling Technology Cat.# 8240), SUZ12 (Cell Signaling Technology Cat.#3737), and RING1B (Cell Signaling Technology Cat.#5694) were

(20) CUL4B orchestrates mesenchymal stem cell commitment by epigenetically repressing KLF4 and C/EBPδBone researchJune 2, 2023Yu R, Han H, Chu S, Ding Y, Jin S, Wang Y, Jiang W, Liu Y, Zou Y, Wang M, et al.anti-EZH2 (CST, 5246 S), anti-HDAC1 (Abcam, ab280198), anti-HDAC3 (CST, 85057 S), anti-H2AK119ub1 (CST, 8240 S), anti-H3K27me3 (Abcam, ab6002), anti-AcH3 (CST, 8173 S), anti-AcH4 (Abcam, ab45166), anti-H2A (Proteintech,

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Ubiquityl-Histone H2A (Lys119) (D27C4) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Ubiquityl-Histone H2A (Lys119) (D27C4) XP ® Rabbit mAb from Cell Signaling Technology

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