Sirtuin 1/SIRT1 Antibody (1F3) [Alexa Fluor® 647] NBP1-51641AF647 from Novus Biologicals, a Bio-Techne Brand

Published Figures

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NPJ Aging
April 19 2022

Fig 1: Schematic diagram of the mechanism of HEI-OC1 mitochondrial biogenesis modulation under H2O2 exposure and PQQ protection.A Sirtuin 1 (SIRT1) expression and deacetylation activity are decreased in HEI-OC1 cells by H2O2 exposure. PGC-1α mediates the protective effect of SIRT1 expression and mitochondrial biogenesis and function. PQQ improves mitochondrial biogenesis and function by restoring SIRT1 expression and deacetylation activity under H2O2 exposure in HEI-OC1 cells. B Schematic diagram of mitochondrial morphological changes.
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NPJ Aging
April 19 2022

Fig 2: Analysis of protein expression and protein acetylation in the premature cellular senescence model under PQQ pretreatment.A SIRT1 and PGC-1α protein expression was analyzed by Western blotting (upper, middle) using β-actin as a loading control (lower). B Relative protein expression level of SIRT1 normalized by β-actin. n = 10 per group. C Relative protein expression level of PGC-1α normalized by β-actin. D Acetylation of PGC-1α analyzed by immunoprecipitation. E Relative acetylation level of PGC-1α normalized by PGC-1α. F Relative mRNA expressions of GAPDH, SIRT1, and PGC-1α using β-actin as an internal control. G The changes of SIRT1 expression in flow cytometry analysis. H The rate of SIRT1 positive cells (B, C, E, H: n = 5 per group, F: n = 3 per group; repeated experiments). Box plot shows statistical parameters as follows; central line: median; box limits: first and third quartile; whiskers: minimum and maximum. RQ data are shown as mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, *****P < 0.00001.