Fig 1: Single-cell transcriptomic analysis reveals IEO cellular diversity(A) Uniform manifold approximation and projection (UMAP) plot showing scRNA-seq and snRNA-seq data of D75-D110 IEOs with cell type annotations.(B) Relative contribution of cell types and large groups.(C) Marker genes for cell type annotation.(D) Skeletal myocyte cluster highlighted by skeletal myocyte marker genes.(E) ACTC1+ TNNT1+ MYOG+ skeletal myocytes in a D75 aggregate. Scale bar, 50 µm.(F) Ependymal cell cluster with marker gene expression.(G) TTR+ SOX2- SOX10- ependymal cells in a D75 aggregate. Scale bar, 50 µm.(H) Endothelial cluster with marker genes.(I) PECAM1+ endothelial cells forming luminal-like structures in a D75 aggregate (I'). Scale bars, 50 µm.POM, periotic mesenchyme. IHC images (E, G, and I) are representative of n = 6 aggregates (=2 experiments). See also Figures S2–S5 and S8.