Fig 1: Chemokines CCL17 and CCL22 are highly expressed in human ‘hot’ tumors and have strong correlation with Treg recruitment. Messenger RNA (mRNA) expression analysis in human tumor patient samples (TCGA database) and normal tissue (GTEX database). Each cross plot indicates a specific type of tumor (left) or tissue (right). (A) Correlation plot of CD8 and FOXP3 expression in tumor (left graph) and normal tissue (right graph). (B) Correlation plot of CCL17+CCL22 and FOXP3 expression in tumor (left graph) and normal tissue (right graph). (C) Representative flow cytometry plots of chemokine receptor expression on CD25+ CD127low Treg in PBMCs from three different donors. CCR4, CC chemokine receptor 4; GTEX, Genotype-Tissue Expression; TCGA, The Cancer Genome Atlas.