Fig 1: Culture and identification of bone marrow mesenchymal stem cells. (A) Light microscopy of BMSCs (scale bar=100 µm for the left image and 200 µm for the right image). (B) Percentage of CD34-, CD45-, CD73-, CD90- and CD105-positive BMSCs were detected by flow cytometry. BMSC, bone marrow mesenchymal stem cell.
Fig 2: Innate type 2 immune responses are exaggerated in Plscr1-null mice.(A) Gating strategy for ILC2 cells in mouse lung. ILC2 are defined as Lin-Thy1.2+ICOS+T1/ST2+ cells. (B) WT and Plscr1–/– mice were subjected to HDM administration (25 µg HDM 3 times a week for 3 weeks), and lung ILC2 recovery was assessed by flow cytometry. (C and D) Whole lung mRNA was extracted and IL-25 mRNA (C) and IL-33 mRNA (D) were assessed by RT-PCR. (E) BAL SLPI levels were measured by ELISA. (F and G) ILC2 cells were isolated as described in methods. mRNA was extracted from primary ILC2s; IL-25R mRNA (F) and IL-33R mRNA (G) were assessed by RT-PCR. (H) BAL soluble IL-33R/ST2 levels were measured by ELISA. Values are mean± SEM with 7–9 mice in each group. Comparisons between groups were conducted by 2-way ANOVA with Bonferroni’s posthoc test. *P = 0.05, **P = 0.01.
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