Fig 2: Effects of Chrono overexpression of skeletal muscle on the amount of Chrono bound to Bmal1 (B), the mRNA expression of Chrono (A), Tbc1d1, Glut4, Hk2, Pfkm, Pdp1 and Pdk4 (C) and Hk activity (D) in skeletal muscle. # p < 0.05, ## p < 0.01, Chrono TG vs. WT.

Fig 3: The effect of Chrono overexpression of skeletal muscle on mRNA expression of genes involved in Gys1, Gbe1, and Phka (A), Gys activity (B), glycogen content (C), and protein expression of Ldha and Ldhb (D) in skeletal muscles. # p < 0.05, ## p < 0.01, Chrono TG vs. WT.

Fig 4: Effects of Chrono overexpression of skeletal muscle on body weight (A), food intake (B), and adipose tissue weight (C).

Fig 5: Effects of Chrono overexpression of skeletal muscle on systemic glucose homeostasis. Glucose tolerance is depicted as blood glucose versus time post-glucose injection (A) and AUC (B). Nonfasted blood glucose (C). Nonfasted blood insulin (D). # p < 0.05, ## p < 0.01 vs. WT.