Fig 1: Effect of insulin therapy on InsB:12-20-reactive CD39- and/or CD73-expressing FoxP3+ Tregs. Frequencies of InsB:12-20-reactive cells within the CD4+FoxP3+CD39+ T cell population (A, D), CD4+FoxP3+CD73+ T cell population (B, E), and CD4+FoxP3+CD39+CD73+ T cell population (C, F) are shown at 15 weeks of age in PLN and/or pancreas of NOD mice. Female NOD mice received four weekly subcutaneous injections, from 10 until 13 weeks of age, of InsB:8-24 peptide formulated in alum (Ins+alum), InsB:8-24 peptide dissolved in buffer (Ins), alum alone (alum), or left untreated (Untr). Data presented as median with interquartile range; symbols (n = 2-6) represent individual mice. *P = 0.05; Treg, regulatory T cell; NOD, non-obese diabetic; PLN, pancreas-draining lymph nodes.