Fig 1: Replenishment of KC Pool by Ly6Chi Monocytes(A and B) Expression of GFP, Ly6C, and F4/80 of monocytes (green gate), em-KCs (black gate), and mo-KCs (red gate) after DT injection in (A) Clec4fDTR/+-Ccr2GFP/+ mice or (B) Clec4fDTR/+-Ccr2GFP/GFP mice. Flow-cytometry plots are pre-gated on live CD45+CD11b+Lyve-1-SiglecF-Ly6G- single cells. Data are representative of 2–3 experiments.(C) Proportion of Ly6Chi monocytes (green lines), em-KCs (black lines), and mo-KCs (red lines) in the liver of Clec4fDTR/+-Ccr2GFP/+ mice (solid lines) or Clec4fDTR/+-Ccr2GFP/GFP mice (dashed lines) as a percentage of live CD45+ cells after DT injection. Pooled data are from 2–3 experiments; n = 5 (0,5d), 6 (PBS, 1d; 1,5d; 2d; 5d; 6d), and 8 mice (3d; 4d).(D) Heatmap showing the top 30 of upregulated genes in mo-KCs 3 days compared with 1 and 7 days after DT injection.(E) Expression of Ki-67 and EdU incorporation by em-KCs and mo-KCs in (top) Clec4fDTR/+-Ccr2GFP/+ mice or (bottom) in Clec4fDTR/+-Ccr2GFP/GFP mice after DT injection. Flow-cytometry plots are pre-gated as in (A). Data are representative of 2–3 experiments.(F) Percentage of EdU+ cells in indicated populations during the differentiation kinetic of mo-KCs in Clec4fDTR/+-Ccr2GFP/+ mice (solid line) or Clec4fDTR/+-Ccr2GFP/GFP (dash line). Pooled data are from 2–3 experiments; n = 5 (0,5d), 6 (PBS, 1d, 1,5d, 2d, 5d, 6d), and 8 mice (3d, 4d). Two-way ANOVA with Tukey post-test. *p < 0.05, ***p < 0.001, ****p < 0.0001.(G) Percentage of EdU+ mo-KCs 3 days after DT injection in liver of mice treated with PLX3397 (a-CSF1R) or vehicle. Pooled data are from 2 experiments, n = 7. Mann-Whitney t test. ***p < 0.001.Related to Figure S1.