Fig 1: Induction of basophil activation by purified Can f 6After non-specific IgEs on basophils being removed, cells from each donor were sensitized with sera from 6 healthy subjects or 6 dog allergic patients, and were challenged with Can f 6 at 1.0 µg/ml. The values shown are mean ±SEM for the sera from 6 different subjects. A. Gating strategy of the basophil by CCR3 and SSC. B. The results of 6 healthy subjects’ sera. C. The results of 6 dog allergic patients’ sera.