Fig 1: Characterization of ECs by flow cytometry markers. The cells were stained for the following markers: VWF, ACE, CD31, CD34, CD133, CD105, PDPN, AP, aSMA, EGFR. Data were recorded for 10,000 events using CellQuest software (v.2.3.0.84) and presented as histogram overlays. (A) The most characteristic markers for ECs and EPCs. (B) Markers associated with cancerous phenotype. Histogram overlays display representative repetitions; gray—healthy ECs unstained, black—tumor ECs unstained, green—healthy ECs stained, red—tumor ECs stained. Y axis = the number of events; X axis = fluorescence intensity; the bar charts present delta MFI. Ns—not significant; * p < 0.05 in Student t test vs. HBH.MEC. Data are reported as the means ± SEM (n = 3).