Fig 1: Flow cytometry-based validation of depleting the contaminating CD45+/CD31+/Ter119+ cell population in cultured metaphyseal/endosteal and neonatal cells. (A) Contour plots showing the gating strategy used for flow cytometry-based quantification of the percentage of skeletal cells (CD51+CD45-CD31-Ter119-) after depletion of CD45-CD31-Ter119- and expansion of purified metaphyseal/endosteal SSPCs in 21% O2 (upper panels) and in 1% O2 (lower panels) (n=3). (B) Contour plots showing the gating strategy used for flow cytometry-based quantification of the percentage of skeletal cells (CD51+CD45-CD31-Ter119-) after depletion of CD45-CD31-Ter119- and expansion of neonatal SSPCs in 21% O2 (upper panels) and in 1% O2 (lower panels) (n=3). Data are shown as mean ± SD.