Fibronectin Antibody Alexa Fluor 488 53-9869-82 from Thermo Fisher Scientific

Product Specs
ItemFibronectin Antibody Alexa Fluor 488
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number53-9869-82
Quantity100 µg
ApplicationsImmunocytochemistry, Immunohistochemistry (Paraffin)
Concentration0.5 mg/mL
Conjugate/TagAlexa Fluor™ 488
FormatPBS with 0.09% sodium azide; pH 7.2
ReactivityHuman
TargetFibronectin
IsotypeIgG1
CloneFN-3
HostMouse
NCBI Full Gene Namefibronectin 1
NCBI Gene AliasesCIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF
ClonalityMonoclonal
PurityAffinity chromatography
Storage Temperature4° C, store in dark, DO NOT FREEZE!
Antibody TypePrimary
Storage4° C, store in dark, DO NOT FREEZE!

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Co-administration of temozolomide (TMZ) and the experimental therapeutic targeting miR-10b, profoundly affects the tumorigenic phenotype of human glioblastoma cellsFrontiers in Molecular BiosciencesJune 15, 2023Ming Chen, Bryan Kim, Neil Robertson, Sujan Kumar Mondal, Zdravka Medarova, Anna Moorewere stained with mouse anti-human Fibronectin Alexa Fluor® 488 (1/100 dilution, Invitrogen Catalog # 53-9869-82). Cells were washed three times for 10 min with DPBS and mounted with VECTASHIELD Antifade Mounting

(2) Multimodal imaging demonstrates enhanced tumor exposure of PEGylated FUD peptide in breast cancerJournal of Controlled ReleaseOctober 1, 2022Hye Jin Lee, Metti K. Gari, David R. Inman, Zachary T. Rosenkrans, Brian M. Burkel, Aeli P. Olson, Jonathan W. Engle, Reinier Hernandez, Suzanne M. Ponik, Glen S. Kwonmatrix, total FN was visualized with an A488-labeled anti-cellular FN monoclonal antibody (eBioscience.No.53–9869–82) diluted in 1% BSA in PBS. Co-localization of A488-FN and Cy5-peptide was visualized by confocal microscopy

(3) Increased Stiffness Downregulates Focal Adhesion Kinase Expression in Pancreatic Cancer Cells Cultured in 3D Self-Assembling Peptide ScaffoldsBiomedicinesJuly 29, 2022Betriu N, Andreeva A, Alonso A, Semino CE.

(4) Transglutaminase 2: Development of therapeutic antibodies reveals four inhibitory epitopes and confirms extracellular function in fibrotic remodellingBritish journal of pharmacologyJune 1, 2022Maamra M, Benayad OM, Matthews D, Kettleborough C, Atkinson J, Cain K, Bon H, Brand H, Parkinson M, Watson PF, et al.

(5) Application of a High-Content Screening Assay Utilizing Primary Human Lung Fibroblasts to Identify Antifibrotic Drugs for Rapid Repurposing in COVID-19 PatientsSLAS discovery : advancing life sciences R & DOctober 1, 2021Marwick JA, Elliott RJR, Longden J, Makda A, Hirani N, Dhaliwal K, Dawson JC, Carragher NO.three times with PBS. The cells were then labeled with Alexa Fluor 488 anti-fibronectin (Ebioscience, 53-9869-82; 1 in 400) for 1.5 h, followed by washing three times with PBS and stored in the dark at 4 °C. High-Content

(6) ILB(®) resolves inflammatory scarring and promotes functional tissue repairNPJ Regenerative medicineJanuary 7, 2021Hill LJ, Botfield HF, Begum G, Qureshi O, Vigneswara V, Masood I, Barnes NM, Bruce L, Logan A.Thermo Fisher Scientific). Cells were then immunostained with mouse anti-human Fibronectin Alexa Fluor® 488 (1/200 dilution, 53-9869-82, Thermo Fisher Scientific) and nuclear stain Hoechst 33342 (H21492, Thermo Fisher Scientific) in permeabilizing

(7) 3D Extracellular Matrix Regulates the Activity of T Cells and Cancer Associated Fibroblasts in Breast CancerFrontiers in oncologyJanuary 1, 2021Gao H, Tian Q, Zhu L, Feng J, Zhou Y, Yang J.

(8) A high content, phenotypic 'scar-in-a-jar' assay for rapid quantification of collagen fibrillogenesis using disease-derived pulmonary fibroblastsBMC biomedical engineeringJanuary 1, 2019Good RB, Eley JD, Gower E, Butt G, Blanchard AD, Fisher AJ, Nanthakumar CB.I (Sigma, C2456), collagen type IV (eBioscience, 50–9871-82) or cellular fibronectin (eBioscience, 53–9869-82) at 1:1000 in PBS for 1.5 h at RT, or overnight at 4 °C. After washing in PBS-Tween (0.05% v/v), cells

(9) Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV IntegrinsNephronJanuary 1, 2019Bon H, Hales P, Lumb S, Holdsworth G, Johnson T, Qureshi O, Twomey BM.

(10) PEGylated pUR4/FUD peptide inhibitor of fibronectin fibrillogenesis decreases fibrosis in murine Unilateral Ureteral Obstruction model of kidney diseasePloS oneJanuary 1, 2018Tomasini-Johansson BR, Zbyszynski PW, Toraason I, Peters DM, Kwon GS.Alexa488-labeled anti cellular FN monoclonal antibody (A488-EDA, eBioscience/Thermo Fisher cat. No. 53-9869-82) or a control antibody, Alexa 488-labeled IgG1, diluted in 2% BSA/PBS to 2.5 μg/ml was added and incubated

(11) An immunofluorescence assay for extracellular matrix components highlights the role of epithelial cells in producing a stable, fibrillar extracellular matrixBiology openOctober 15, 2017Qureshi OS, Bon H, Twomey B, Holdsworth G, Ford K, Bergin M, Huang L, Muzylak M, Healy LJ, Hurdowar V, et al.

(12) Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironmentOncoimmunologyJanuary 1, 2017Jiang X, Seo YD, Chang JH, Coveler A, Nigjeh EN, Pan S, Jalikis F, Yeung RS, Crispe IN, Pillarisetty VG.

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Fibronectin Antibody Alexa Fluor 488 from Thermo Fisher Scientific

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Fibronectin Antibody Alexa Fluor 488 from Thermo Fisher Scientific

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