Myosin 4 Antibody 14-6503-82 from Thermo Fisher Scientific

Product Specs
ItemMyosin 4 Antibody
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number14-6503-82
Quantity100 µg
ApplicationsImmunocytochemistry, Western Blot
Concentration0.5 mg/mL
Conjugate/TagUnconjugated
FormatPBS with 0.09% sodium azide; pH 7.2
ReactivityBovine, Canine, Chicken, Chimpanzee, Feline, Guinea Pig, Human, Mouse, Non-human primate, Rabbit, Rat
TargetMyosin 4
IsotypeIgG2b kappa
CloneMF20
HostMouse
NCBI Full Gene Namemyosin, heavy polypeptide 4, skeletal muscle, myosin heavy chain 4
NCBI Gene AliasesMYH4, MM, MyHC-2B, MYH2B, Myhsf, MYH-2B, MHC2B, MHC, MyHC-IIb, Minmus, Minimsc
ClonalityMonoclonal
PurityAffinity chromatography
Storage Temperature4° C
Antibody TypePrimary
Storage4° C

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Investigating proliferation and differentiation capacities of Hanwoo steer myosatellite cells at different passages for developing cell-cultured meatScientific ReportsSeptember 20, 2023Sanghun Park, Gyutae Park, Sehyuk Oh, Yunhwan Park, Yuna Kim, Jaeyoung Kim, Jungseok Choimice (1:3000, Sigma-Aldrich, Cat # A2228), myosin 4 monoclonal antibody (1:1000, Invitrogen, Cat # 14-6503-82), Tom20 (F-10) (1;1000, Santa Cruz, Cat # sc-17764). After washing three times for 10 min with TBST

(2) Melatonin Improves Skeletal Muscle Structure and Oxidative Phenotype by Regulating Mitochondrial Dynamics and Autophagy in Zücker Diabetic Fatty RatAntioxidants (Basel)July 27, 2023Diego Salagre, Enrique Raya Álvarez, Cruz Miguel Cendan, Samira Aouichat, Ahmad Agilor in mouse against MyH1 (cat#MBS252930; MyBioSource), MyH2 (cat#MABT840; Sigma-Aldrich), MyH4 (cat#14-6503-82; Thermo Scientific) at 1:1000 dilutions in Blocking Buffer. Primary anti-β-actin antibody generated

(3) LBP1C-2 from Lycium barbarum maintains skeletal muscle satellite cell pool by interaction with FGFR1iScienceMay 19, 2023Meng J, Lv Z, Chen X, Sun C, Jin C, Ding K, Chen C.goat anti-rabbit IgG antibody,Invitrogen,Cat#F-2765; RRID: AB_2536525 Anti-MyHC (MF20),Invitrogen,Cat#14-6503-82; RRID: AB_2572894 Anti-FGFR1 (phospho Y653) antibody [EPR843(N)],Abcam,Cat# ab111124; RRID: AB_10859625 Anti-FGFR1 antibody [EPR806Y

(4) The developing epicardium regulates cardiac chamber morphogenesis by promoting cardiomyocyte growthDisease Models & MechanismsMay 1, 2023Giulia L. M. Boezio, Shengnan Zhao, Josephine Gollin, Rashmi Priya, Shivani Mansingh, Stefan Guenther, Nana Fukuda, Felix Gunawan, Didier Y. R. StainierRoche), anti-dsRed (rabbit, 1:200; cat. no. 632496, Takara), and anti-MF20 (mouse, 1:500; cat. no. 14-6503-82, Invitrogen) primary antibodies at 4°C overnight. After several washes with PBS containing 0.1% Tween-20,

(5) Endothelial Brg1 fine-tunes Notch signaling during zebrafish heart regenerationnpj Regenerative MedicineApril 7, 2023Chenglu Xiao, Junjie Hou, Fang Wang, Yabing Song, Jiyuan Zheng, Lingfei Luo, Jianbin Wang, Wanqiu Ding, Xiaojun Zhu, Jing-Wei Xiong(A-11122; Invitrogen; 1:500), anti-PCNA (P8825; Sigma; 1:300), anti-myosin heavy-chain monoclonal antibody (14-6503-82; eBioscience; 1:500), anti-DsRed (BE3307; EASYBIO; 1:300) and the Brg1 antibody (1:250), which was raised

(6) Multiple pkd and piezo gene family members are required for atrioventricular valve formationNature CommunicationsJanuary 13, 2023Thomas Juan, Agatha Ribeiro da Silva, Bárbara Cardoso, SoEun Lim, Violette Charteau, Didier Y. R. Stainierprimary antibodies anti-DIG-POD (1:5000, Roche 11207733910) and anti-MHC (MF20) (1:500, Invitrogen 14-6503-82) diluted in PBST + 10% Western Blocking reagent (Roche 11921673001). On the third day, after six washes

(7) Mice with R2509C-RYR1 mutation exhibit dysfunctional Ca2+ dynamics in primary skeletal myocytesJournal of General PhysiologyNovember 7, 2022Yoshitaka Tsuboi, Kotaro Oyama, Fuyu Kobirumaki-Shimozawa, Takashi Murayama, Nagomi Kurebayashi, Toshiaki Tachibana, Yoshinobu Manome, Emi Kikuchi, Satoru Noguchi, Takayoshi Inoue, et al.the cells were incubated overnight at 4°C with mouse–anti-myosin heavy chain antibody (MF20, 1:200; 14-6503-82; Thermo Fisher Scientific). After washing, the cells were incubated with Alexa Fluor 546 rabbit anti-mouse IgG (H+L

(8) Severe congenital myasthenic syndromes caused by agrin mutations affecting secretion by motoneuronsActa neuropathologicaOctober 1, 2022Jacquier A, Risson V, Simonet T, Roussange F, Lacoste N, Ribault S, Carras J, Theuriet J, Girard E, Grosjean I, et al.Developmental Studies Hybridoma Bank, DSHB; for IF 1:50); mouse monoclonal anti-heavy chain of myosin II, MF20 (# 14-6503-82; ThermoFisher Scientific; for IF 1:500); the clusters of AchRs were labeled with Alexa Fluor 488/555-conjugated

(9) Irisin Preserves Cardiac Performance and Insulin Sensitivity in Response to HemorrhagePharmaceuticals (Basel)September 27, 2022Supaporn Kulthinee, Lijiang Wang, Naohiro Yano, Patrycja M. Dubielecka, Ling X. Zhang, Shougang Zhuang, Gangjian Qin, Yu Tina Zhao, Yue Eugene Chin, Ting C. Zhaodark. After washing with PBS, a mouse monoclonal anti-MF-20 antibody (1:200, Thermo Fisher Scientific #14-6503-82) was added and incubated for two hours at room temperature. After sections were washed three times with

(10) Long non-coding RNA Tug1 modulates mitochondrial and myogenic responses to exercise in skeletal muscleBMC biologyJuly 18, 2022Trewin AJ, Silver J, Dillon HT, Della Gatta PA, Parker L, Hiam DS, Lee YP, Richardson M, Wadley GD, Lamon S.Calbiochem, 1:1000), anti-Myosin Skeletal-Slow/MyHC-i (M8421, Sigma, 1:5000), anti-Myosin 4/MyHC-iib (14-6503-82, eBioscience/Thermo, 1:1000), anti-citrate synthase (14309, Cell Signaling Technology, 1:1000), anti-OPA1

(11) Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured MeatBiomoleculesJune 16, 2022Park S, Gagliardi M, Swennen G, Dogan A, Kim Y, Park Y, Park G, Oh S, Post M, Choi J.antibody produced in mice (1:3000, Cat: A2228, Sigma-Aldrich), myosin 4 monoclonal antibody (1:1000, Cat: 14-6503-82, Invitrogen), Tom20 Antibody (F-10) (1:1000, Cat: sc-17764, Santa Cruz). After washing three times with

(12) Electroporation of Small Interfering RNAs into Tibialis Anterior Muscles of MiceBio-protocolJune 5, 2022Stephan A, Graca FA, Hunt LC, Demontis F.

(13) The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophyNature communicationsMay 2, 2022Graca FA, Rai M, Hunt LC, Stephan A, Wang YD, Gordon B, Wang R, Quarato G, Xu B, Fan Y, et al.with rFibcd1). Myotubes were then fixed and stained for myosin heavy chain (MF20 clone, eBioscience, #14-6503-82; used at 1:150) and myotube diameters analyzed by ImageJ, as explained in detail below. For the analysis

(14) Primary cilia on muscle stem cells are critical to maintain regenerative capacity and are lost during agingNature communicationsMarch 17, 2022Palla AR, Hilgendorf KI, Yang AV, Kerr JP, Hinken AC, Demeter J, Kraft P, Mooney NA, Yucel N, Burns DM, et al.catalog #H00011116-M01, clone 2B1, 1:1000), anti-MyHC (clone MF20, Thermo Fisher Scientific, catalog # 14-6503-82, 1:500) and then with AlexaFluor secondary Antibodies (Jackson ImmunoResearch Laboratories, 1:500).

(15) Local fluid shear stress operates a molecular switch to drive fetal semilunar valve extensionDevelopmental DynamicsMarch 1, 2022Duc H. Pham, Charles R. Dai, Belle Y. Lin, Jonathan T. Butcherovernight at 4C. The primary antibodies used were PHH3 (1:200; Abcam, ab10543), MF20 (1:100; Thermo Fisher, 14-6503-82), pSmad1/5 (1:100; Cell Signaling, 9516S), and mouse mAb against β-catenin (1:100; BD Biosciences; 610154).

(16) Sumoylation-deficient phosphoglycerate mutase 2 impairs myogenic differentiationFrontiers in cell and developmental biologyJanuary 1, 2022Zhang Y, Beketaev I, Ma Y, Wang J.(Centennial, CO, United States). The anti-sarcomeric α-myosin heavy chain (α-MHC) antibody MF20 (Cat#14-6503-82, 1:500) was obtained from eBioscience, Inc. (San Diego, CA, United States). HRP-conjugated HA antibody

(17) Engineered whole cut meat-like tissue by the assembly of cell fibers using tendon-gel integrated bioprintingNature communicationsAugust 24, 2021Kang DH, Louis F, Liu H, Shimoda H, Nishiyama Y, Nozawa H, Kakitani M, Takagi D, Kasa D, Nagamori E, et al.finally 300 nM DAPI (Invitrogen, D21490) counterstaining. Myosin 4 Monoclonal Antibody (eBioscience, 14-6503-82, MF20, dilution 1/500) for bSCs, Anti-CD31 (Wako, M0823, JC70A, dilution 1/100) for bovine endothelial cells

(18) Hydrostatic mechanical stress regulates growth and maturation of the atrioventricular valveDevelopment (Cambridge, England)July 1, 2021Bassen D, Wang M, Pham D, Sun S, Rao R, Singh R, Butcher J.

(19) TAM kinase signaling is indispensable for proper skeletal muscle regeneration in miceCell death & diseaseJune 12, 2021Al-Zaeed N, Budai Z, Szondy Z, Sarang Z.

(20) Nanopore direct RNA sequencing detects DUX4-activated repeats and isoforms in human muscle cellsHuman molecular geneticsMay 12, 2021Mitsuhashi S, Nakagawa S, Sasaki-Honda M, Sakurai H, Frith MC, Mitsuhashi H.immunostained against primary antibodies (mouse monoclonal IgG2 anti-myosin heavy chain antibody, eBioscience, 14-6503-82; rabbit monoclonal anti-MyoD antibody, Abcam, ab133627; mouse monoclonal IgG1 anti-myogenin antibody,

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Myosin 4 Antibody from Thermo Fisher Scientific

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Myosin 4 Antibody from Thermo Fisher Scientific

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