Fig 2: Autophagy inhibitors can reverse the impact of PBM on H2O2-induced MC3T3 cells. (a–c) Western blotting assays of the LC3B and P62 expression levels of in the H2O2, 3-MA, and treated with PBM groups (n = 4). (d–f) The levels of Bax and Bcl2 expressions in the H2O2, 3-MA, and treated with PBM groups (n = 4) were measured by western blotting. (g, h) Western blotting was performed to assay the levels of Runx2, Osx, OPN, and OCN in the H2O2, 3-MA, and treated with PBM groups (n = 4). (i) Alphabetic staining and alizarin red staining of MC3T3 cells processed with H2O2, 3-MA, and PBM. The p values < 0.05, 0.01, and 0.001 and the scale bar represents 200 µm.