Fig 2: Th1-like Transcriptomic Patterns(A) Heatmap defines meta-clusters based on Pearson correlation among TIL, dLN, and Arm cluster fold change vectors (as defined in the text) (left). Tables show tissue origin and cell-type color code per cluster (right).(B and C) Comparison of TIL Th1 and Isc (clusters t1–2 and t3–4, respectively, as shown in Figure 1A), as well as Arm Th1 (as shown in Figures 2 and S2A).(B) Contour plots of Th1 (orange) and Isc (blue) TIL distribution according to scRNA-seq-detected normalized expression of Irf7 versus Ifit3b (left) and Klrc1 versus Lag3 (right).(C) Heatmap shows row-standardized expression of differentially expressed genes across TIL group II Isc, TIL group I Th1, and Arm Th1.(D) (Left) Flow cytometry contour plots of NKG2A versus CD94 (top) or IRF7 (bottom) in Foxp3-GP66+ dLN, TIL, and Arm cells. (Right) Percentage of NKG2A+CD94+ cells (top) and IRF7hi NKG2A- cells (bottom) among Foxp3-GP66+ CD4+ T cells; each symbol represents an individual mouse.(E) Overlaid protein expression of T-bet in NKG2A+ and NKG2A- Foxp3-GP66+ TILs (left). The graph on the right summarizes quantification (mean fluorescence intensity, MFI) of T-bet in each subset, expressed relative to naive CD4+ splenocytes from tumor-free control mice. Each symbol represents an individual mouse; lines indicate pairing.(F) Flow cytometry contour plots of T-bet versus IRF7 in Foxp3-GP66+ dLN, TILs, and Arm cells; data from naive CD4+ splenocytes from tumor-free control mice is shown as a control (right plot).(D–F) Each plot is representative from 10 tumor-bearing and 9 Arm-infected mice, analyzed in two separate experiments. Each symbol on summary graphs represents one mouse.(G) (Left) Overlaid protein expression of IFN? in NKG2A+ versus NKG2A- TILs and Arm cells. Data are shown for Foxp3-GP66+ cells (plain lines); expression on Foxp3+ cells is shown as a negative control (shaded gray). (Right) Graph shows the percentage of IFN?+ cells out of NKG2A+ or NKG2A- Foxp3- TILs or of GP66+ Arm CD4+ T cells and summarizes a single experiment with 5 tumor-bearing and 3 Arm-infected mice. Data are representative of two such experiments, with 15 tumor-bearing and 5 Arm-infected mice. Each symbol on summary graphs represents one mouse.Two-tailed unpaired (D and G) or paired (E) t test; *p < 0.05, **p < 0.01, and ****p < 0.0001.See also Figure S3 and Table S2.