Fig 1: Identification of the PLPs expressing the protein complexes in platelet aggregation assays. (A) A quantity of 200 µL PRP was mixed well with 200 µL WT PLPs. The mixed sample was divided equally into two groups: one was stained with the FITC-conjugated CD41a antibody, and the other was stained with the APC-conjugated CD42b antibody. After staining, the two groups were mixed again thoroughly, and AA was added to induce aggregation (3 µM). Then, the sample was analyzed by flow cytometry. About 67% of aggregation was recognized. (B) A quantity of 200 µL PRP was mixed completely with 200 µL PLPs expressing SC-TP-Gαq. Then, the above procedures were repeated. The flow cytometry analysis indicated about 74% of aggregation. (C) The mixture of 200 µL PRP and 200 µL PLPs expressing SC-TP-Gαs was used to repeat the above steps. Around 9% of aggregation was demonstrated in this group. (D) Statistical analysis of platelet aggregation. * p < 0.05 *** p < 0.001.