Fig 2: IL-12p70 variability in healthy donors(A–E) IL-12p70 (A) measured by Luminex in healthy donors (n = 1,000) after stimulation of whole blood with null, LPS, or poly (I:C). IL-12p40 (B) and IL-23 (C) proteins measured by Luminex in healthy donors (n = 1,000) after null and LPS stimulation. IL-12p70 secretion measured by Simoa after null and LPS stimulation at two separate time points (visit 1 n = 1,000, D; visit 2 n = 500, E).(F) Correlation of IL-12p70 secretion after LPS 22 h stimulation at two separate time points. Pearson correlation was performed on log10 values.(G) IL-12p70 secretion measured by Simoa in the two groups of Milieu Interieur IL-12p70 responders after null and E. coli stimulation. Red lines indicate the median value for each group. p values were determined by an unpaired Student’s t test using log10 values.(H) Correlation of IL-12p70 secretion measured by Simoa after E. coli and LPS 22 h stimulation. Pearson correlation was performed using log10 values. Empty symbols represent IL-12p70 low responders as defined by LPS 22 h whole-blood stimulation (A); full symbols represent IL-12p70 responders.

Fig 3: CD14+ monocytes and dendritic cells secrete IL-12p70 after LPS stimulation(A) Representative dot plots of IL-12p70 and IL-23 secretion upon null and LPS 22 h stimulation measured by flow cytometry in CD14+ monocytes, cDC2, and pDCs.(B–D) Tukey box-whisker plots of the percentage of IL-12p70+ cells (B), IL-23+ cells (C), or IL-12p70+ and IL-23+ cells (n = 10; D) for the indicated immune cell populations in null and LPS conditions. Q values were determined by the Wilcoxon test and false discovery rate corrected for multiple comparison testing.(E) Numbers of CD14hi monocytes, cDC2, and pDCs for the responder and low-responder groups (n = 1,000).(F) Expression levels of HLA-DR in CD14+ monocytes and cDC2 populations and CD86 in CD14+ monocytes for the two groups.Red lines indicate the median value for each group. Q values were determined by an unpaired Student’s t test (on log10 values) and false discovery rate corrected for 79 cell numbers (E) and 88 cell surface protein phenotypes (F) in multiple comparison testing (n = 1,000).