Fig 1: The number of CD19+ immunoreactive B cells was greater in slices from females but not males upon treatment with exogenous calcitonin gene‐related peptide (CGRP). (a–c) Male PCLS after exposure to CGRP for 24 or 48 h compared to vehicle. (d–f) Female PCLS after exposure to CGRP for 24 or 48 h compared to the vehicle. (g) CGRP fibers (green) colocalized with B cells (red). (h) B cell numbers were significantly greater in slices taken from females after 24 h of exposure to CGRP compared to the vehicle and greater than B cell numbers in slices from males. No difference was seen at 48 h compared to the vehicle, regardless of the sex of the slice origin. B cell numbers in slices from males did not differ significantly with CGRP treatment. Yellow and white arrows indicate B cells. Dark gray indicates male, light gray indicates female, and bars are paired as 24 h of treatment with CGRP, 48 h of treatment with CGRP, or vehicle. Twenty‐four hours CGRP‐male n = 5 mice/8 PCLS; 24 h CGRP‐female n = 5 mice/9 PCLS; 48 h CGRP‐male n = 4 mice/8 PCLS; 48 h CGRP‐female n = 4 mice/6 PCLS; vehicle‐male n = 6 mice/9 PCLS; vehicle‐female n = 12 mice/24 PCLS. Scale bars are 20 μm in (a–f) and 100 μm in (g).